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The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene

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Abstract

The expression of the Penicillium griseoroseum plg1 gene is induced by citric pectin and repressed by glucose. In this work, the minimal region of the plg1 gene promoter essential for expression in pectin and sucrose plus yeast extract was identified by using constructs containing the gfp ORF under control of the plg1 gene promoter. The fragment A (283 bp) is essential for plg1 expression in sucrose plus yeast extract. Fragment B (309 bp plus 184; core promoter) was critical for expression in pectin and abolished the catabolic repression by glucose. Therefore, the fragment of 776 bp (fragment A and B) is essential for the expression of the plg1 gene in natural inducing conditions (pectin as carbon source) and in sucrose plus yeast extract. The fragment B is a promising minimal promoter usable for heterologous expression in filamentous fungi, since genes that contain it could be activated by the presence of peel from citric fruits (which contains citric pectin) and are not affected by glucose in these agricultural by-products.

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References

  • Alaña A, Llama MJ, Serra JL (1991) Purification and some properties of the pectin lyase from Penicillium italicum. FEBS Lett 280:335–340

    Article  PubMed  Google Scholar 

  • Baracat MC, Valentim C, Muchovej JJ, Silva DO (1989) Selection of pectinolytic fungi for degumming of natural fibers. Biotechnol Lett 11:899–902

    Article  CAS  Google Scholar 

  • Bazzolli DMS, Ribon AOB, Queiroz MV, Araújo EF (2006) Molecular characterization and expression profiling of pectin lyase-encoding genes from Penicillium griseoroseum. Can J Microbiol 52:1070–1077

    Article  CAS  PubMed  Google Scholar 

  • Bussink HJD, Brower KB, De Graaff LH, Kester HCM, Visser J (1991) Identification and characterization of a second polygalacturonase gene of Aspergillus niger. Curr Genet 20:301–307

    Article  CAS  PubMed  Google Scholar 

  • Cardoso PG, Teixeira JA, Queiroz MV, Araújo EF (2010) Pectin lyase production by recombinant Penicillium griseoroseum strain 105. Can J Microbiol 56(10):831–837

    Article  CAS  PubMed  Google Scholar 

  • de Vries RP, Jansen J, Aguilar G, Parenicová L, Joosten V, Wülfert F, Benen JAE, Visser J (2002) Expression profiling of pectinolytic genes from Aspergillus niger. FEBS Lett 530:41–47

    Article  PubMed  Google Scholar 

  • Fawole OB, Odunfa SA (2003) Some factors affecting production of pectic enzymes by Aspergillus niger. Int Biodeterior Biodegrad 52:223–227

    Article  CAS  Google Scholar 

  • Felenbok B, Flipphi M, Nikolaev I (2001) Ethanol catabolism in Aspergillus nidulans: a model system for studying gene regulation. Prog Nucleic Acid Res Mol Biol 69:149–204

    Article  CAS  PubMed  Google Scholar 

  • Glinka EM, Liao Y (2011) Purification and partial characterisation of pectin methylesterase produced by Fusarium asiaticum. Fungal Biol 115:1112–1121

    Article  CAS  PubMed  Google Scholar 

  • Gonçalves DB, Teixeira JA, Bazzolli DMS, Queiroz MV, Araújo EF (2012) Use of response surface methodology to optimize production of pectinases by recombinant Penicillium griseoroseum T20. Biocat Agric Biotechnol 1:140–146

    Google Scholar 

  • Gummadi SN, Kumar DS (2005) Microbial pectic transeliminases. Biotechnol Lett 27:451–458

    Article  CAS  PubMed  Google Scholar 

  • Jayani RS, Saxena S, Gupta R (2005) Microbial pectinolytic enzymes: a review. Process Biochem 40:2931–2944

    Article  CAS  Google Scholar 

  • Minussi RC, Soares-Ramos JRL, Coelho JLC, Silva DO (1998) Sugar-cane juice induces pectin lyase and polygalacturonase in Penicillium griseoroseum. Rev Microbiol 29:246–250

    Article  CAS  Google Scholar 

  • Pereira JF, Queiroz MV, Lopes FJF, Rocha RB, Daboussi MJ, Araújo EF (2004) Characterization, regulation and phylogenetic analyses of the Penicillium griseoroseum nitrate reductase gene and its use as selection marker for homologous transformation. Can J Microbiol 50:891–900

    Article  CAS  PubMed  Google Scholar 

  • Pontecorvo G, Roper JA, Hemmons LM, MacDonald KD, Bufton AW (1953) The genetics of Aspergillus nidulans. Adv Genet 5:141–238

    Article  CAS  PubMed  Google Scholar 

  • Takashima S, Iikura H, Nakamura A, Masaki H, Uozumi T (1996) Analysis of Cre1 binding sites in the Trichoderma reesei cbh1 upstream region. FEMS Microbiol Lett 145:361–366

    Article  CAS  PubMed  Google Scholar 

  • Teixeira JA, Gonçalves DB, Queiroz MV, Araújo EF (2011) Improved pectinase production in Penicillium griseoroseum recombinant strains. J Appl Microbiol 111:818–825

    Article  CAS  PubMed  Google Scholar 

  • Yadav S, Yadav PK, Yadav D, Yadav KDS (2008) Purification and characterization of pectin lyase produced by Aspergillus terricola and its application in retting of natural fibers. Appl Biochem Biotechnol 159(1):270–283. doi:10.1007/s12010-008-8471-1

    Article  PubMed  Google Scholar 

  • Ziemiński K, Romanowska I, Kowalska M (2012) Enzymatic pretreatment of lignocellulosic wastes to improve biogas production. Waste Manag 32:1131–1137

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by grants from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG).

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Authors and Affiliations

  1. Departamento de Microbiologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil

    Denise Mara Soares Bazzolli, Klédna Constância Portes Reis, Janaina Aparecida Teixeira, Marisa Vieira de Queiroz & Elza Fernandes de Araújo

  2. Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, Brazil

    Andréa Oliveira Barros Ribon

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  1. Denise Mara Soares Bazzolli
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  2. Klédna Constância Portes Reis
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  3. Janaina Aparecida Teixeira
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  4. Andréa Oliveira Barros Ribon
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  5. Marisa Vieira de Queiroz
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Correspondence to Elza Fernandes de Araújo.

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Bazzolli, D.M.S., Reis, K.C.P., Teixeira, J.A. et al. The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene. Ann Microbiol 65, 1145–1148 (2015). https://doi.org/10.1007/s13213-014-0873-4

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  • DOI: https://doi.org/10.1007/s13213-014-0873-4

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