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Lysinibacillus composti sp. nov., isolated from compost

Annals of Microbiology volume 64, pages 1081–1088 (2014)Cite this article

Abstract

A Gram-negative, motile, rod-shaped, endospore-forming bacterial strain, designated as NCCP-36T, was isolated from the compost of fruit and vegetable wastes. The strain NCCP-36T grew within a temperature range of 10–45 ○C (optimum 28 ○C) and a pH range of 6.5–8.5 (optimum 7.0), and its cells tolerated <50 mM boron (optimum growth without boron) and 0–5 % NaCl (w/v) in tryptic soya broth medium. Based on comparative analysis of 16S rRNA gene sequence, strain NCCP-36T showed the highest similarity to Lysinibacillus sinduriensis BLB-1T (97.52 %) and L. xylanilyticus XDB9T (96.96 %), and <97 % similarity with other closely related taxa. However, DNA–DNA relatedness between strain NCCP-36T and the closely related type strains of genus Lysinibacillus was ≤37 %. Phylogenetic and chemotaxonomic analyses [major polar lipids: diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and phospholipids; predominant menaquinone: MK-7; major cellular fatty acids: iso-C15:0, antieso-C15:0, and iso-C16:0; DNA G+C contents: 37 mol %; Lys-Asp (type A4α) in cell-wall peptidoglycans as diagnostic amino acids] also support the affiliation of strain NCCP-36T to genus Lysinibacillus. Based upon DNA–DNA relatedness as well as distinctive chemotaxonomic, phylogenetic, and genotypic data, we conclude that strain NCCP-36T belongs to a novel species of genus Lysinibacillus, for which the name Lysinibacillus composti sp. nov. is proposed. The type strain is NCCP-36T (JCM 18777T = KCTC 13796T = DSMZ 24785T).

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Acknowledgments

We gratefully acknowledge the kind help of Dr. Takuji Kudo from JCM, Tsukuba, Japan in the analysis of cell-wall peptidoglycans. This work was partially supported by PSDP Project “Research for Agricultural Development Program (RADP)” funded by Pakistan Agricultural Research Council (PARC) and by the KRIBB Research Initiative Program funded by the Ministry of Education, Science and Technology, Republic of Korea. Initial funds for these studies were provided by the Higher Education Commission of Pakistan under talent training program for young faculty members for training at National Institute for Genomics and Advanced Biotechnology, NARC, Islamabad, Pakistan and also at the Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.

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Authors and Affiliations

  1. National Culture Collection of Pakistan (NCCP), National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre (NARC), Park Road, Islamabad, 45500, Pakistan

    Rifat Hayat, Iftikhar Ahmed, Muhammad Ehsan & Muhammad Iqbal

  2. Department of Soil Science & SWC, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, 46300, Pakistan

    Rifat Hayat & Muhammad Ehsan

  3. Korean Collection for Type Cultures, Biological Resources Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, Republic of Korea

    Jayoung Paek, Yeseul Sin & Young H. Chang

  4. Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-Ku, Tokyo, 113-8657, Japan

    Rifat Hayat & Akira Yokota

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  1. Rifat Hayat
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  2. Iftikhar Ahmed
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  3. Jayoung Paek
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Correspondence to Iftikhar Ahmed or Young H. Chang.

Additional information

The DDBJ/EMBL/GenBank accession number for the 16S rRNA gene sequence of strain NCCP-36T (=JCM 18777T = KCTC 13796 T; DSMZ 24785 T) is AB547124.

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Hayat, R., Ahmed, I., Paek, J. et al. Lysinibacillus composti sp. nov., isolated from compost. Ann Microbiol 64, 1081–1088 (2014). https://doi.org/10.1007/s13213-013-0747-1

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Keywords

  • Lysinibacillus composti sp. nov.
  • Compost
  • Cell-wall peptidoglycans