Abstract
An antagonistic bacterium, denoted as strain Z01, was isolated from suppressive soils and identified using fatty acid profile analysis and molecular tools. Its efficacy to control Fusarium oxysporum f. sp. conglutinans on rockets (cv. Eruca sativa) was investigated in the presence of the pathogen, and its effects on the growth and pigment contents of rockets, including the plant biomass and chlorophyll and carotenoid contents, were evaluated in the absence of the pathogen. Z01 was identified as Pantoea agglomerans (GenBank accession number is JX257179). Rockets were grown under greenhouse conditions for 21 days at 25 °C and 95 % relative humidity. When the antagonist suspension was applied at 108, 107 and 106 cells/ml by root-dipping for 20 min before transplanting, the disease incidence was 23.5 %, 28.5 % and 60.0 %, respectively. The disease incidence of rockets treated with carbendazim was 22.5 %, and in the inoculated control, the disease incidence was 82.0 %. In the absence of the pathogen, compared with the average plant biomass (10.7 g/4 plants) of the uninoculated control, application of the antagonist at 108 or 107 cells/ml significantly increased the biomass of the rockets by 40.9 % and 39.1 %, respectively, suggesting that Z01 had an additional ability to promote plant growth beyond its role in Fusarium wilt control. This study indicated that P. agglomerans Z01 has a potential to control Fusarium wilt in rockets. This report is the first to describe the application of P. agglomerans to control Fusarium wilt.
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Acknowledgments
The work was supported by the Science and Technology Innovation Fund from Beijing Academy of Agriculture and Forestry Sciences (No. QNJJ201317), and by the Science and Technology Plan Project of Beijing (No. Z121100001212002), and also by Special Research Fund for Agro-Scientific Research in the Public Interest from the Chinese Agriculture Ministry (No. 200903049-07).
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Liu, Wc., Chen, Zc., Zhang, Tt. et al. Application of Pantoea agglomerans strain Z01 to control Fusarium wilt and its effect on the quality parameters of rockets. Ann Microbiol 64, 1443–1446 (2014). https://doi.org/10.1007/s13213-013-0735-5
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DOI: https://doi.org/10.1007/s13213-013-0735-5