Abstract
When inoculated in association with Saccharomyces cerevisiae, the yeast Lachancea thermotolerans determines a reduction of volatile acidity and an increase in the production of glycerol, 2-phenylethanol, and polysaccharides. Moreover, L. thermotolerans is a natural L-lactic acid producer, thus it contributes to wine acidification and microbiological stabilization. In view of its utilization in winemaking, a culture-independent PCR-based method was developed for the detection of L. thermotolerans during wine fermentations. This method, which utilizes species-specific PCR primer pairs that anneal to intron 2 of the mitochondrial COX1 gene, is rapid and reliable, and detects L. thermotolerans in wine at 104 cells/ml and with a S. cerevisiae/L. termotholerans ratio of 1,000/1.
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Comitini F, Gobbi M, Domizio P, Romani C, Lencioni L, Mannazzu I, Ciani M (2011) Selected non-Saccharomyces wine yeasts in controlled multistarter fermentations with Saccharomyces cerevisiae. Food Microbiol 28:873–882
Foury F, Roganti T, Lecrenier N, Purnelle B (1998) The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae. FEBS Lett 440:325–331
Gobbi M, Comitini F, Domizio P, Romani C, Lencioni L, Mannazzu I, Ciani M (2013) Lachancea thermotolerans and Saccharomyces cerevisiae in simultaneous and sequential co-fermentation: a strategy to enhance acidity and improve the overall quality of wine. Food Microbiol 33:271–281
Lopez V, Fernández-Espinar MT, Barrio E, Ramón D, Querol A (2003) A new PCR-based method for monitoring inoculated wine fermentations. Int J Food Microbiol 81:63–71
Mannazzu I, Angelozzi D, Belviso S, Budroni M, Farris GA, Goffrini P, Lodi T, Marzona M, Bardi L (2008) Behaviour of Saccharomyces cerevisiae wine strains during adaptation to unfavourable conditions of fermentation on synthetic medium: cell lipid composition, membrane integrity, viability and fermentative activity. Int J Food Microbiol 121:84–91
Molitor C, Inthavong B, Sage L, Geremia RA, Mouhamadou B (2010) Potentiality of the COX1 gene in the taxonomic resolution of soil fungi. FEMS Microbiol Lett 302:76–84
Mouhamadou B, Carriconde F, Gryta H, Jargeat P, Manzi S, Gardes M (2008) Molecular evolution of mitochondrial ribosomal DNA in the fungal genus Tricholoma: barcoding implications. Fungal Gen Biol 45:1219–1226
Seifert KA, Samson RA, Dewaard JR, Houbraken J, Lévesque CA, Moncalvo JM, Louis-Seize G, Herbert PD (2007) Prospect for fungus identification using COX1 barcodes, with Penicillium as a test case. Proc Natl Acad Sci USA 104:3901–3906
Talla E, Anthouard V, Bouchier C, Frangeul L, Dujon B (2005) The complete mitochondrial genome of the yeast Kluyveromyces thermotolerans. FEBS Lett 579:30–40
Ushinsky SC, Bussey H, Ahmed AA, Wang Y, Friesen J, Williams BA, Storms RK (1997) Histone H1 in Saccharomyces cerevisiae. Yeast 13:151–161
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Zara, G., Ciani, M., Domizio, P. et al. A culture-independent PCR-based method for the detection of Lachancea thermotolerans in wine. Ann Microbiol 64, 403–406 (2014). https://doi.org/10.1007/s13213-013-0647-4
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DOI: https://doi.org/10.1007/s13213-013-0647-4