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Purification and characterization of antifungal compounds from Bacillus coagulans TQ33 isolated from skimmed milk powder

Abstract

Bacillus coagulans TQ33, isolated from skimmed milk powder, displays strong antifungal activity against plant pathogenic fungi. The antifungal compound of the B. coagulans TQ33 culture was extracted by thin-layer chromatography and column chromatography, and its structure was elucidated based on HPLC, LC-MS, and NMR analysie. The antifungal compound was identified as phenyllactic acid (PLA), and it was found to have a minimum inhibitory concentration on Phytophthora drechsleri Tucker of 18 mg/mL. Bio-control activity tests indicated that PLA has a wide spectrum of antagonistic effects against Fusarium oxysporum, Botrytis cinerea, Glomerella cingulata, Penicillium citrinum, Penicillium digitatum, particularly against F. oxysporum. PLA is the most notable antimicrobial compound with broad and effective antimicrobial activity against both bacteria and fungi that has been isolated and identified to date. These results indicate that B. coagulans TQ33 has the potential for application in biological pesticides.

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Acknowledgments

Financial support provided by the National Natural Science Foundation of China (No. 30900961 and No. 20876116) and TianJin Social Science Planning Program (No. TJGLWT11-08) is gratefully acknowledged.

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Correspondence to HaiKuan Wang.

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Wang, H., Shen, F., Xiao, R. et al. Purification and characterization of antifungal compounds from Bacillus coagulans TQ33 isolated from skimmed milk powder. Ann Microbiol 63, 1075–1081 (2013). https://doi.org/10.1007/s13213-012-0564-y

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  • DOI: https://doi.org/10.1007/s13213-012-0564-y

Keywords

  • Plant pathogenic fungi
  • Antifungal activity
  • Bacillus coagulans TQ33
  • Phenyllactic acid