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Active metabolites produced by Penicillium chrysogenum IFL1 growing on agro-industrial residues

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Abstract

Microbial extracts continue to be a productive source of new molecules with biotechnological importance. Fungi of the genus Penicillium are known to produce biologically active secondary metabolites. The goal of this work is verify the production of antimicrobial metabolites by Penicillium chrysogenum IFL1 using agro-industrial residues. P. chrysogenum IFL1 produced active metabolites growing on the agro-industrial residues, grape waste and cheese whey. The 7-day cultures showed antimicrobial activities against bacteria, fungi and amoebae. The filtrate of the cheese whey culture inhibited the growth of the bacteria Staphylococcus aureus, Bacillus cereus and Pseudomonas aeruginosa, the fungus Fusarium oxysporum and the amoeba Acanthamoeba polyphaga. Due to the greater antimicrobial activity of the cheese whey culture, a footprinting profile was carried out using the ESI-MS and ESI-MS/MS techniques. The presence of penicillin G and other metabolites that have antimicrobial activity such as penicillin V and rugulosin can be suggested. P. chrysogenum IFL1 was able to produce a wide variety of antimicrobial compounds on agro-industrial residues, which makes the process ecologically friendly.

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Acknowledgments

Authors thank Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for financial support. In addition, we also thank Dr. Patricia Valente da Silva, Dr. Charley Christian Staats, Dr. Fernanda Stanisçuaski (UFRGS) and Dr. João Lúcio de Azevedo (ESALQ/USP) for the insightful suggestions on the manuscript.

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Authors and Affiliations

  1. Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos (ICTA), Universidade Federal do Rio Grande do Sul, Av Bento Gonçalves 9500, 91501-970, Porto Alegre, Brazil

    Fernanda C. Lopes, Deise M. Tichota, Stela M. M. Meira & Adriano Brandelli

  2. Laboratório de Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

    Ismael P. Sauter & Marilise B. Rott

  3. Unidade Química de Proteínas e Espectrometria de Massas (UNIPROTE-MS), Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

    Jeferson Segalin

  4. Laboratório de Análise de Alimentos, Departamento de Ciência de Alimentos (ICTA), Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

    Alessandro O. Rios

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  1. Fernanda C. Lopes
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  2. Deise M. Tichota
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  3. Ismael P. Sauter
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  4. Stela M. M. Meira
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  6. Marilise B. Rott
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  7. Alessandro O. Rios
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  8. Adriano Brandelli
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Corresponding author

Correspondence to Adriano Brandelli.

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Lopes, F.C., Tichota, D.M., Sauter, I.P. et al. Active metabolites produced by Penicillium chrysogenum IFL1 growing on agro-industrial residues. Ann Microbiol 63, 771–778 (2013). https://doi.org/10.1007/s13213-012-0532-6

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  • DOI: https://doi.org/10.1007/s13213-012-0532-6

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