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Prevalence, distribution and antibiotic resistance pattern among enterococci species in two traditional fermented dairy foods

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Abstract

The prevalence, distributions and antibiotic resistance pattern among enterococci species were determined. A total of 30 samples of Nigerian traditional fermented dairy food were positive to presence of enterococci, with viable counts of 4.17 log CFU/g in nunu, lower than 4.55 log CFU/g observed in wara samples. Twenty-five representative strains were characterized by a combination of phenotypic and genomic typing based on 16S rRNA gene and multi-locus sequencing analysis (MLSA) of RNA polymerase A (rpoA) and phenylanaline synthase (pheS) genes sequencing; these strains were identified as Enterococcus faecium (84 %) and Enterococcus faecalis (16 %). All the 95 enterococci isolated from wara and nunu samples were alpha haemolytic with multi-drug resistance to 10 antimicrobials regardless of class. Four strains were sensitive to chloramphenicol (30 μg) while 33.7 % of the total isolates were resistant to vancomycin from 5 μg. This information will enhance understanding of Enterococcus drug resistance and distribution in traditional fermented foods to support safety and guarantee quality of traditional foods in West Africa.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Science, University of Lagos, Akoka, Lagos, Nigeria

    Folarin Anthony Oguntoyinbo & Olayinka Okueso

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  1. Folarin Anthony Oguntoyinbo
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  2. Olayinka Okueso
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Correspondence to Folarin Anthony Oguntoyinbo.

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Oguntoyinbo, F.A., Okueso, O. Prevalence, distribution and antibiotic resistance pattern among enterococci species in two traditional fermented dairy foods. Ann Microbiol 63, 755–761 (2013). https://doi.org/10.1007/s13213-012-0530-8

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  • DOI: https://doi.org/10.1007/s13213-012-0530-8

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