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Annals of Microbiology

, Volume 63, Issue 2, pp 683–689 | Cite as

Quick identification and quantification of Proteus mirabilis by polymerase chain reaction (PCR) assays

  • Weiwei Zhang
  • Zongliang Niu
  • Kun Yin
  • Ping Liu
  • Lingxin ChenEmail author
Original Article

Abstract

Proteus mirabilis is an opportunistic pathogen that can cause urinary tract infection in human beings. The accurate and rapid identification and quantification of P. mirabilis is necessary for early treatment. In this study, a pair of specific primers according to the conserved ureR sequence of P. mirabilis was designed and novel systems which consisted of a polymerase chain reaction (PCR) and a real-time PCR to identify and quantify P. mirabilis were developed. For the qualitative identification by ordinary PCR, a 225-bp DNA product was amplified from P. mirabilis and separated on an agarose gel. The corresponding DNA product is present in three P. mirabilis strains isolated from different geographical locations, but is absent in 20 strains representing 18 different species, including the ureR homolog contained Providencia stuartii and Escherichia coli strains, the other common pathogens Klebsiella sp., Edwarsiella sp., Vibrio sp., Enterobacter sp., and Escherichia sp., and other environmental bacteria Pseudomonas sp. and Acinetobacter sp. Proteus mirabilis at concentrations higher than 1.0 × 103 CFU ml−1 was detectable by ordinary PCR; P. mirabilis at concentrations higher than 10 CFU ml−1 was quantified by real-time PCR. The specific, sensitive and time-efficient PCR methods were demonstrated to be applicable to rapid identification and quantification of P. mirabilis.

Keywords

Proteus mirabilis ureR Polymerase chain reaction (PCR) Real-time PCR 

Notes

Acknowledgements

The authors are grateful for the financial support provided by the Innovation Projects of the Chinese Academy of Sciences grant KZCX2-EW-206, the National Natural Science Foundation of China (NSFC) grant 20975089, the Department of Science and Technology of Yantai City of China grant 2010235, the Doctoral Foundation of Shandong Province grant BS2011SW056, and the 100 Talents Program of the Chinese Academy of Sciences.

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Copyright information

© Springer-Verlag and the University of Milan 2012

Authors and Affiliations

  • Weiwei Zhang
    • 1
    • 2
  • Zongliang Niu
    • 1
    • 2
  • Kun Yin
    • 1
    • 2
    • 3
  • Ping Liu
    • 1
    • 2
  • Lingxin Chen
    • 1
    • 2
    • 4
    Email author
  1. 1.Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantaiPeople’s Republic of China
  2. 2.Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantaiPeople’s Republic of China
  3. 3.Graduate University of the Chinese Academy of SciencesBeijingChina
  4. 4.Yantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantaiChina

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