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Statistical optimization of glucose oxidase production from Aspergillus niger NRC9 under submerged fermentation using response surface methodology

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Abstract

Response surface methodology (RSM), employing the fractional factorial design (FFD) was used to optimize the fermentation medium for the production of glucose oxidase (GOD) from a marine isolate (NRC9) of Aspergillus niger under submerged fermentation. The design was employed by selecting glucose, CaCO3, ammonium phosphate and MgSO4 concentrations as model factors by ‘one variable at a time’ experiment. A second-order quadratic model and response surface method showed that the optimum concentrations (g/l) glucose, 100; CaCO3, 25; (NH4)2HPO4, 1.8 and 0.4 of MgSO4, resulted in an improvement of GOD production (170 ± 0.88 U/ml) as compared to the initial level (109.81 ± 1.38 U/ml) after four days of incubation at 200 rpm and 30 °C, whereas its predicted value obtained by the quadratic model was 164.36 U/ml. Analysis of variance (ANOVA) showed a high coefficient of determination value (R 2) of 0.967, ensuring a satisfactory adjustment of the quadratic model with the experimental data. This is the first report on production of glucose oxidase from a marine fungal isolate, Aspergillus niger NRC9, using statistical experimental design and response surface methodology in optimization of its production under submerged fermentation.

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Acknowledgments

This work was supported by National Research Center, Chemistry of natural and microbial products department (Egypt).

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Authors and Affiliations

  1. Chemistry of Natural and Microbial Products Department, National Research Center, Dokki, Cairo, Egypt

    Mohammed A. Farid, Asmaa Negm-Eldein & Ghada E. A. Awad

  2. Plant and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt

    Eglal A. Ghoneimy & Maie A. El-Khawaga

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  1. Mohammed A. Farid
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  2. Eglal A. Ghoneimy
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  3. Maie A. El-Khawaga
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  4. Asmaa Negm-Eldein
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  5. Ghada E. A. Awad
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Correspondence to Ghada E. A. Awad.

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Farid, M.A., Ghoneimy, E.A., El-Khawaga, M.A. et al. Statistical optimization of glucose oxidase production from Aspergillus niger NRC9 under submerged fermentation using response surface methodology. Ann Microbiol 63, 523–531 (2013). https://doi.org/10.1007/s13213-012-0497-5

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  • DOI: https://doi.org/10.1007/s13213-012-0497-5

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