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Annals of Microbiology

, Volume 63, Issue 1, pp 71–79 | Cite as

Investigation on diversity and population succession dynamics of endophytic bacteria from seeds of maize (Zea mays L., Nongda108) at different growth stages

  • Yang Liu
  • Shan Zuo
  • Yuanyuan Zou
  • Jianhua WangEmail author
  • Wei SongEmail author
Original Article

Abstract

Plant seeds are carriers of both beneficial bacteria and pathogens. Using the 16S rRNA gene clone library technique, we conducted a preliminary study on the community diversity and population succession dynamics of endophytic bacteria in seeds of reciprocal cross hybrid maize at different seed developmental stages. In both hybrid lines (108A and 108B), more types of endophytic bacteria were found at the proembryo-forming stage than in the other two stages, including 29 and 23 bacterial operational taxonomic units (OTUs), respectively. Undibacterium (39.20 and 30.00 % in 108A and 108B, respectively) was the first dominant bacterium to appear. At the milky stage, fewer types of endophytic bacteria in 108A and 108B appeared, including 18 and 16 OTUs, respectively, and the abundance of the dominant genus Burkholderia in the two seed samples reached 73.38 and 80.43 %, respectively. Limnobacter appeared as the second and third endophytic dominant bacterium in 108A (4.55 %) and 108B (5.07 %), respectively, in both seed samples. At the dough stage, the abundance of the first dominant bacterium, Burkholderia, in 108A and 108B was 78.26 and 84.80 %, respectively. Pantoea appeared as the second endophytic dominant bacterium in the both seeds (9.42 and 4.80 % in 108A and 108B, respectively). This is the first study on endophytic bacteria present during several crucial stages of the dynamic grain growth process of plant seeds conducted using culture-independent methods.

Keywords

Reciprocal cross maize seed Endophytic bacteria Bacterial diversity Population succession 16S rRNA gene library 

Abbreviation

CTAB

Cetyltrimethylammonium bromide

PGPB

Plant growth-promoting bacteria

Notes

Acknowledgments

We would like to thank Professor Yueming Yan at Capital Normal University and Doctor Fengge Wang at Beijing Academy of Agriculture and Forestry Sciences for their assistance in providing genetic knowledge of hybrid maize. This work was supported by the National Natural Science Foundation of China (No. 30770069) and Science Foundation of Beijing (No. 5092004). We would also like to thank Christine Verhille at the University of British Columbia for her assistance with English language and grammatical editing of the manuscript.

References

  1. Adams PD, Kloepper JW (2002) Effect of host genotype on indigenous bacterial endophytes of cotton (Gossypium hirsutum L.). Plant Soil 240:181–189CrossRefGoogle Scholar
  2. Bacilio-Jiméne M, Aguilar-Flores S, del Valle MV, Pérez A, Zepeda A, Zenteno E (2001) Endophytic bacteria in rice seeds inhibit early colonization of roots by Azospirillum brasilense. Soil Biol Biochem 33:167–172CrossRefGoogle Scholar
  3. Barea JM, Pozo MJ, Azcon R, Azcon-Aguilar C (2005) Microbial co-operation in the rhizosphere. J Exp Bot 56:1761–1778PubMedCrossRefGoogle Scholar
  4. Cankar K, Kraigher H, Ravnikar M, Rupnik M (2005) Bcterial endophytes from seeds of Norway spruce (Picea abies L. Karst). FEMS Microbiol Lett 244:341–345PubMedCrossRefGoogle Scholar
  5. Chun J, Lee JH, Jung Y, Kim M, Kim S, Kim BK, Lim YW (2007) EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261PubMedCrossRefGoogle Scholar
  6. Coombs JT, Franco CM (2003) Visualization of an endophytic Streptomyces species in wheat seed. Appl Environ Microbiol 69:4260–4262PubMedCrossRefGoogle Scholar
  7. Cottyn B, Regalado E, Lanoot B, De Cleene M, Mew TW, Swings J (2001) Bacterial populations associated with rice seed in the tropical environment. Phytopathology 91:282–292PubMedCrossRefGoogle Scholar
  8. Cui BB, Li Y, Jin XJ, Feng H (2010) Cytological mechanism of cytoplasmic inheritance in section Leuce (I): Cytoplasmic DNA within generative cell and sperm cell. J Beijing For Univ 32:54–62Google Scholar
  9. Ferreira A, Quecine MC, Lacava PT, Oda S, Azevedo JL, Araújo WL (2008) Diversity of endophytic bacteria from Eucalyptus species seeds and colonization of seedlings by Pantoea agglomerans. FEMS Microbiol Lett 287:8–14PubMedCrossRefGoogle Scholar
  10. Gitaitis R, Walcott R (2007) The epidemiology and management of seedborne bacterial diseases. Annu Rev Phytopathol 45:371–397PubMedCrossRefGoogle Scholar
  11. Glick BR, Patten CL, Holguin G, Penrose DM (1999) Biochemical and genetic mechanism used by plant growth-promoting bacteria. Imperial College Press, LondonCrossRefGoogle Scholar
  12. Good IJ (1953) The population frequencies of species and the estimation of population parameters. Biometrika 40:237–264Google Scholar
  13. Grum M, Camloh M, Rudolph K, Ravnikar M (1998) Elimination of bean seed-borne bacteria by thermotherapy and meristem culture. Plant Cell Tissue Org Cult 52:79–82CrossRefGoogle Scholar
  14. Guan KL (2009) Seed physiological ecology (in Chinese). China Agriculture Press, BeijingGoogle Scholar
  15. Hu J (2006) Seed Biology (in Chinese). Higher Education Press, BeijingGoogle Scholar
  16. Johnston-Monje D, Raizada MN (2011) Conservation and diversity of seed associated endophytes in Zea across boundaries of evolution, ethnography and ecology. PLoS One 6(6):e20396. doi: 10.1371/journal.pone.0020396 PubMedCrossRefGoogle Scholar
  17. Kloepper JW, Beauchamp CJ (1992) A review of issues related to measuring colonization of plant roots by bacteria. Can J Microbiol 38:1219–1232CrossRefGoogle Scholar
  18. Kutschera U (2002) Bacterial colonization of sunflower cotyledons during seed germination. J Appl Bot 76:96–98Google Scholar
  19. Majewska-Sawka A, Nakashima H (2004) Endophyte transmission via seeds of Lolium perenne L.: immunodetection of fungal antigens. Fungal Genet Biol 41:534–541PubMedCrossRefGoogle Scholar
  20. Mano H, Tanaka F, Watanabe A, Kaga H, Okunishi S, Morisaki H (2006) Culturable surface and endophytic bacterial flora of the maturing seeds of rice plants (Oryza sativa) cultivated in a paddy field. Microbes Environ 21:86–100CrossRefGoogle Scholar
  21. Mavingui P, Laguerre G, Berge O, Heulin T (1992) Genetic and phenotypic diversity of Bacillus polymyxa in soil and in the wheat rhizosphere. Appl Environ Microbiol 58:1894–1903PubMedGoogle Scholar
  22. Michiels K, Vanderleyden J, Gool A (1989) Azospirillum–plant root associations: a review. Biol Fertil Soils 8:356–368CrossRefGoogle Scholar
  23. Mullins TD, Britschgi TB, Krest RL, Giovannoni SJ (1995) Genetic comparisons reveal the same unknown bacterial lineages in atlantic and pacific bacterioplankton communities. Limnol Oceanogr 40:148–158CrossRefGoogle Scholar
  24. Mundt JO, Hinkle NF (1976) Bacteria within ovules and seeds. Appl Environ Microbiol 32(5):694–698PubMedGoogle Scholar
  25. Neal JL, Larson RI, Atkinson TG (1973) Changes in rhizosphere populations of selected physiological groups of bacteria related to substitution of specific pairs of chromosomes in spring wheat. Plant Soil 39:209–212CrossRefGoogle Scholar
  26. Nelson EB (2004) Microbial dynamics and interactions in the spermosphere. Annu Rev Phytipathool 42:271–309CrossRefGoogle Scholar
  27. Okunishi S, Sako K, Mano H, Imamura A, Morisaki H (2005) Bacterial flora of endophytes in the maturing seed of cultivated rice (Oryza sativa). Microbes Environ 20:168–177CrossRefGoogle Scholar
  28. Rijavec T, Lapanje A, Dermastia M, Rupnik M (2007) Isolation of bacterial endophytes from germinated maize kernels. Can J Microbiol 53:802–808PubMedCrossRefGoogle Scholar
  29. Simon HM, Smith KP, Dodsworth JA, Guenthner B, Handelsman J, Goodman RM (2001) Influence of tomato genotype on growth of inoculated and indigenous bacteria in the spermosphere. Appl Environ Microbiol 67:514–520PubMedCrossRefGoogle Scholar
  30. Song W, Yang HL, Sun XL, Wang YS, Wang YD, Chen ZH (1998) The rice endophytic diazotroph and PGPR. In: Malik KA, Mirza MS, Ladha JK (eds) Nitrogen fixation with non-legumes. Academic Publishers/Kluwer, DordrechtGoogle Scholar
  31. Song SQ, Cheng HY, Jiang XC (2008) Seed Biology (in Chinese). Science Press, BeijingGoogle Scholar
  32. Sun L, Qiu F, Zhang X, Dai X, Dong X, Song W (2008) Endophytic bacterial diversity in rice (Oryza sativa L.) roots estimated by 16S rDNA sequence analysis. Microb Ecol 55:415–424PubMedCrossRefGoogle Scholar
  33. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version4.0. Mol Biol Evol 24:1596–1599PubMedCrossRefGoogle Scholar
  34. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680PubMedCrossRefGoogle Scholar
  35. Wang RD, Yin JZ (2005) Crop Cultivation Science (in Chinese). Higher Education Press, BeijingGoogle Scholar
  36. Xie ZW, Song GE, Hong DY (1999) Preparation of DNA from silica gel dried mini-amount of leaves of Oryza rufipogon for RAPD study and total DNA bank construction. Acta Bot Sin 41:807–812Google Scholar

Copyright information

© Springer-Verlag and the University of Milan 2012

Authors and Affiliations

  1. 1.College of Life SciencesCapital Normal UniversityBeijingPeople’s Republic of China
  2. 2.College of Agriculture and BiotechnologyChina Agricultural UniversityBeijingPeople’s Republic of China
  3. 3.China Center of Industrial Culture CollectionChina National Research Institute of Food and Fermentation IndustriesBeijingPeople’s Republic of China

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