Biosynthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from Bacillus cereus FA11 isolated from TNT-contaminated soil
- 322 Downloads
In the present study, a poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [poly(3HB-co-3HV)]-producing bacterial strain was isolated from trinitrotoluene (TNT)-contaminated soil using Sudan black B and Nile blue A staining as a screening method. The bacterial strain was identified as Bacillus cereus FA11 on the basis of biochemical tests and 16S rRNA sequencing. Culture conditions, such as pH, temperature, time and carbon sources, were optimized for maximum production of the copolymer. B. cereus FA11 was found capable of producing a maximum of 48.43% poly(3HB-co-3HV) at pH 7, with glucose as the carbon source, after 48 h of incubation at 30°C. Fourier transform infrared analysis of the extracted polymer revealed similarities in the spectra of the microbial and commercially available poly(3HB-co-3HV) at 1720.2, 1278.5 and 2932.1 cm−1 wavelength. 1H-nuclear magnetic resonance analysis of the polymer confirmed it as a copolymer composed of 3-hydroxybutyrate and 3-hydroxyvalerate monomeric units.
KeywordsTNT-contaminated soil Biodegradable thermoplastic Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Bacillus cereus
We thank Higher Education Commission (HEC), Pakistan, for providing financial assistance to complete this research work.
- Arnold L, Demain J, Davis E (1999). Polyhydroxyalkanoates. Manual of microbiology and biotechnoloy, vol. 2. American Society of Microbiology, Washington D.C., pp 616–627Google Scholar
- Arshad MU, Jamil N, Naheed N, Husnain S (2007) Analysis of bacterial strains from contaminated and non-contaminated sites for the production of biopolymers. Afr J Biotechnol 6:1115–1121Google Scholar
- Eck RV, Dayhoff MO (1966) Atlas of protein sequence and structure. National Biomedical Research Foundation, Silver SpringsGoogle Scholar
- Holt JG (1993) Bergey’s manual of determinative bacteriology, 9th edn. Lippincott William and Wilkins, BaltimoreGoogle Scholar
- Kulkarni SO, Kanekar PP, Nilegaonkar SS, Sarnaik SS, Jog JP (2010) Production and characterization of a biodegradable poly (hydroxybutyrate-co-hydroxyvalerate) (PHB-co-PHV) copolymer by moderately haloalkalitolerant Halomonas campisalis MCM B-1027 isolated from Lonar Lake India. Bioresource Technol 101(24):9765–9771CrossRefGoogle Scholar
- Lee SY, Choi JIL (1999) Polyhydroxyalkanoates: Biodegradable polymer. In: Demain AL, Davies JE (eds) Manual of industrial microbiology and biotechnology, 2nd edn. ASM Press, Washington D.C. pp 616–627Google Scholar
- Lemoigne M (1923) Production d'acide β-oxybutyrique par certaines bactéries du groupe B. subtilis. C R Acad Sci 176:1761–1765Google Scholar
- Liu H, Pancholi M, Stubbs J III, Raghavan D (2010) Influence of hydroxyvalerate composition of polyhydroxybutyrate valerate (PHBV) copolymer on bone cell viability and in vitro degradation. J Appl Polym Sci 116(6):3225–3231Google Scholar
- Ojumu TV, Yu J, Solomon BO (2004) Production of polyhydroxyalkonates, a bacterial biodegradable polymer. Afr J Biotechnol 3:18–24Google Scholar
- Weisberg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:(2)697–703Google Scholar
- Yüksekdağ ZN, Aslim B, Beyatli Y, Mercan N (2004) Effect of carbon and nitrogen sources and incubation times on poly-beta-hydroxybutyrate (PHB) synthesis by Bacillus subtilis 25 and Bacillus megaterium 12. Afr J Biotechnol 3:63–66Google Scholar