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Viability of Agaricus blazei after long-term cryopreservation

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Abstract

Agaricus blazei is a basidiomycete of medicinal and gastronomic importance, but few publications have appeared on preservation. We have evaluated A. blazei cryopreservation at −70°C using different cryoprotectants and freezing protocols. Malt extract agar disks containing grown mycelia were transferred to cryotubes containing different cryoprotective solutions. Freezing protocols were from 25°C to: (1) 4°C for 30 min and then to −70°C or (2) directly to −70°C. The results demonstrate that it is the cryoprotective agent—and not the freezing protocol—which was the most important variable for maintaining mycelial viability after cryopreservation. Long-term cryopreservation (4 years) was effective when saccharose or glucose was used as cryoprotectant regardless of the freezing protocol, dimethyl sulfoxide was effective when a slow freezing protocol was used, and glycerol, polyethylene glycol, and malt extract were ineffective as cryoprotectants regardless of the freezing protocol. All of the cryoprotectants tested were effective for the short-term cryopreservation (1 year) of A. blazei, with the exception of malt extract.

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Acknowledgments

The authors thank the Universidade Paranaense and the Master’s Program in Biotechnology Applied to Agriculture.

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Authors and Affiliations

  1. Molecular Biology Laboratory, Universidade Paranaense, 87.502-210, Umuarama-PR, Brazil

    Nelson Barros Colauto, Fábio Aparecido Cordeiro, Kassia Vanessa Navarro Geromini, Tatiana Gomes de Lima, Ana Daniela Lopes, Renato Alessandro Ribeiro Nunes, Fernando de Brito Roratto, Henrique Susumu Tanaka, Lienine Luiz Zaghi Jr. & Giani Andrea Linde

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  1. Nelson Barros Colauto
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  2. Fábio Aparecido Cordeiro
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Correspondence to Nelson Barros Colauto.

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Colauto, N.B., Cordeiro, F.A., Geromini, K.V.N. et al. Viability of Agaricus blazei after long-term cryopreservation. Ann Microbiol 62, 871–876 (2012). https://doi.org/10.1007/s13213-011-0368-5

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