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Bacterial diversity in the polluted water of the Dianchi Lakeshore in China

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Abstract

Dianchi Lake is a typical Chinese eutrophic lake. The bacterial community in the polluted water of the Dianchi lakeshore was investigated by cultivation-independent approaches. The amplicon length heterogeneity polymerase-chain reaction (LH-PCR) was used to detect the major differences in bacterial structure among the nine different sampling sites. Cluster analysis shows that the bacterial communities in water blooming sites were similar. Three genes were employed to characterize bacteria in the two parts of Dianchi Lake. The 16S rRNA gene was used to analyze the total bacterial community, the ammonia monooxygenase (amoA) gene for detecting ammonia-oxidizing bacteria, and the nitrous oxide reductase (nosZ) gene for identifying denitrifying bacteria. The clone library results demonstrate that Proteobacteria, Bacteroidetes, and Cyanobacteria were the dominant bacteria in both parts of the lake. The communities of ammonia-oxidizing bacteria were very different in the two parts of the lake and belonged to Nitrospira in the north and Nitrosomonas in the south part, respectively. Denitrifying bacteria in the Dianchi lakeshore were related to several cultured denitrifiers such as Pseudomonas, Paracoccus, Achromobacter, and Rubrivivax.

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Acknowledgments

This study was supported by a General Project (No. 51008003) grant from the National Natural Science Foundation of China and a National Science & Technology Mega-program on Water Pollution Control for the 11th Five-Year Plan (2009ZX07102-002-01). The authors thank Dr. Zbigniew Cichacz in the Biodesign Institute at Arizona State University for carefully checking of this paper.

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Authors and Affiliations

  1. College of Environmental Sciences and Engineering, The Key Laboratory of Water and Sediment Sciences (Ministry of Education), Peking University, Beijing, 100871, People’s Republic of China

    Donghui Wen, Yaohui Bai, Qinghua Sun, Renhua Sun & Xiaoyan Tang

  2. School of Water Resource and Environmental Science, China University of Geosciences (Beijing), Beijing, 100083, People’s Republic of China

    Qing Shi & Chuanping Feng

  3. Kunming Institute of Environmental Science, Kunming, Yunnan Province, 650032, People’s Republic of China

    Zongxun Li

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  1. Donghui Wen
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  2. Yaohui Bai
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  3. Qing Shi
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  4. Zongxun Li
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  5. Qinghua Sun
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  6. Renhua Sun
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  7. Chuanping Feng
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  8. Xiaoyan Tang
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Correspondence to Donghui Wen or Yaohui Bai.

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Wen, D., Bai, Y., Shi, Q. et al. Bacterial diversity in the polluted water of the Dianchi Lakeshore in China. Ann Microbiol 62, 715–723 (2012). https://doi.org/10.1007/s13213-011-0311-9

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  • DOI: https://doi.org/10.1007/s13213-011-0311-9

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