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Screening of a xylanase high-producing strain and its rapid separation and purification

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Abstract

In this research, the xylanase high-producing strain of BE-91 (Bacillus subtilis) was selected. The enzyme activity in the fermentation liquor of BE-91 at 8 h reached 408 U/mL, which was 3.4 times higher than that of strain ACCC 10243. The xylanase was purified from BE-91 fermentation liquor with the ultrafiltration and gel chromatography, and its enzyme activity was up to 28,454 U/mg. Its recovery was above 69%, and the purification multiple of the enzyme activity was up to 18 times. The molecular weight of the purified xylanase was 22.54 kDa assayed with SDS-PAGE. The Km and Vmax were 0.5 mg/mL and 533 μmol/(min mL), respectively. The stabilizing pH and optimal pH of the xylanase were 4.6~6.4 and 5.8, respectively. And when the pH was 5.8, the stabilizing temperature and optimal temperature of the xylanase were 0°C~65°C and 60°C, respectively. Therefore it was considered that the strain BE-91 could be applied to the industrial production of xylanase.

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Acknowledgment

This work was financially supported by the the National High Technology Research and Development Program of China (863 Program, No. 2006AA02Z249), and National Acro-Industry Techenology Research System for Bast and Leaf Fiber Crops (nycytx-19-E21).

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Authors and Affiliations

  1. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan, 410205, People’s Republic of China

    Zhengchu Liu, Shengwen Duan, Ke Zheng, Xiangyuan Feng, Lifeng Cheng & Jun Shi

  2. Hunan Agricultural University, Changsha, Hunan, 410128, People’s Republic of China

    Xiaoyang Dai

  3. Huaihua University, Huaihua, 418000, Hunan, People’s Republic of China

    Juzuo Zhang & Junfei Xu

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  1. Zhengchu Liu
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  2. Xiaoyang Dai
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  3. Juzuo Zhang
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  4. Junfei Xu
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  5. Shengwen Duan
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  6. Ke Zheng
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  7. Xiangyuan Feng
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  8. Lifeng Cheng
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  9. Jun Shi
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Correspondence to Zhengchu Liu.

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Zhengchu Liu and Xiaoyang Dai contributed equally to this work.

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Liu, Z., Dai, X., Zhang, J. et al. Screening of a xylanase high-producing strain and its rapid separation and purification. Ann Microbiol 61, 901–906 (2011). https://doi.org/10.1007/s13213-011-0212-y

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  • DOI: https://doi.org/10.1007/s13213-011-0212-y

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