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16S rRNA gene sequence analysis of halophilic and halotolerant bacteria isolated from a hypersaline pond in Sichuan, China

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Abstract

One hundred and twenty bacterial isolates were obtained from a hypersaline pond (c. 22% salinity) in Sichuan, China. Bacteria were isolated from hypersaline water, sediment and soil samples using three culture media and an incubation temperature of 37°C. Of these isolates, 47 were selected and examined by phylogenetic analysis of 16S rRNA gene sequences and by tests of salt tolerance. The phylogenetic analysis placed the 47 bacterial isolates either in the phylum Firmicutes or in the class Gammaproteobacteria and showed that they were affiliated with the genera Salimicrobium, Halalkalibacillus, Virgibacillus, Alkalibacillus, Marinococcus, Halobacillus, Halomonas, Idiomarina, Chromohalobacter and Halovibrio. All tested isolates were either halophilic or halotolerant and several were capable of growth in the presence of 30% (w/v) NaCl.

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Acknowledgment

This study was supported by a grant from the National High Technology Research and Development Program of China (Program 863; No. 2006AA02Z189).

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Authors and Affiliations

  1. Rice Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan, China, 611130

    Jie Tang, Ai-ping Zheng, Jun Zhu, Shuang-cheng Li, Shi-quan Wang, Qi-ming Deng & Ping Li

  2. Key Laboratory of Southwest Crop Gene Resource & Genetic Improvement of Ministry of Education, Sichuan Agricultural University, Ya’an, Sichuan, China, 625014

    Jie Tang, Ai-ping Zheng, Jun Zhu, Shuang-cheng Li, Shi-quan Wang, Qi-ming Deng & Ping Li

  3. Agriculture and Agri-Food Canada, 960 Carling Ave, Ottawa, Ontario, Canada, K1A 0C6

    Jie Tang & Eden S. P. Bromfield

  4. Rice Research Institute, Dongbei Road No. 555, Liucheng Town, Wenjiang, 611130, Sichuan, People’s Republic of China

    Ping Li

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  1. Jie Tang
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  2. Ai-ping Zheng
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  3. Eden S. P. Bromfield
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  4. Jun Zhu
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  5. Shuang-cheng Li
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  6. Shi-quan Wang
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  7. Qi-ming Deng
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  8. Ping Li
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Correspondence to Ping Li.

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Tang, J., Zheng, Ap., Bromfield, E.S.P. et al. 16S rRNA gene sequence analysis of halophilic and halotolerant bacteria isolated from a hypersaline pond in Sichuan, China. Ann Microbiol 61, 375–381 (2011). https://doi.org/10.1007/s13213-010-0137-x

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  • DOI: https://doi.org/10.1007/s13213-010-0137-x

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