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Effect of abiotic stress conditions on expression of the Lactobacillus brevis IOEB 9809 tyrosine decarboxylase and agmatine deiminase genes

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Abstract

Lactobacillus brevis IOEB 9809, isolated from red wine, is able to produce both tyramine and putrescine. Using a real-time quantitative reverse transription PCR (qRT-PCR) we analyzed the relative expression of L. brevis IOEB 9809 tdc and aguA1 genes, encoding, respectively, a tyrosine decarboxylase and an agmatine deiminase enzyme. Relative gene expression was monitored either during the different growth phases under optimal conditions or under abiotic stress commonly found in wine. The tdc and aguA1 specific cDNA were amplified with specific primers. Our results indicate that tdc and aguA1 genes are differently expressed during the different growth phases and transiently induced by ethanol (12% v/v) and acidic stresses (pH 3.2 and 5.0).

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Acknowledgements

Lactobacillus brevis IOEB 9809 was kindly provided by Professor Patrick Lucas Faculty of Oenology, Université de Bordeaux of Bordeaux. This work was supported by the EU commission in the framework of the BIAMFOOD project (Controlling Biogenic Amines in Traditional Food Fermentations in Regional FP7– project number 211441).

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Authors and Affiliations

  1. Department of Food Science, Foggia University, via Napoli \25, 71100, Foggia, Italy

    Mattia Pia Arena, Pasquale Russo, Vittorio Capozzi, Luciano Beneduce & Giuseppe Spano

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  1. Mattia Pia Arena
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  2. Pasquale Russo
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  3. Vittorio Capozzi
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  4. Luciano Beneduce
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  5. Giuseppe Spano
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Corresponding author

Correspondence to Giuseppe Spano.

Additional information

This paper is part of the special issue "WINE MICROBIOLOGY AND SAFETY: FROM THE VINEYARD TO THE BOTTLE (MICROSAFETYWINE), 19-20 November 2009, Martina Franca (Italy)"

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Arena, M.P., Russo, P., Capozzi, V. et al. Effect of abiotic stress conditions on expression of the Lactobacillus brevis IOEB 9809 tyrosine decarboxylase and agmatine deiminase genes. Ann Microbiol 61, 179–183 (2011). https://doi.org/10.1007/s13213-010-0089-1

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  • DOI: https://doi.org/10.1007/s13213-010-0089-1

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