Abstract
In this study, the development of a duplex PCR for the specific detection of Aspergillus carbonarius—a potent ochratoxin A (OTA) producer—is described. This approach resulted in a very accurate and sensitive method for the detection of low amounts of the mycotoxigenic mould occurring in grapes. In addition, an optimised protocol for the isolation of genomic DNA from a polyphenol-rich fruit like grape was developed.
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Acknowledgements
The authors would like to thank P. Lucia and G. Mosca for technical assistance and E. Pace for providing grape fruits.
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This paper is part of the special issue “Wine microbiology and safety: from the vineyard to the bottle (Microsafetywine)”, 19–20 November 2009, Martina Franca (Italy).
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Lopardo, R., Musto, M., Cellini, F. et al. Development and application of a duplex PCR for the detection of Aspergillus carbonarius occurring in grapes. Ann Microbiol 61, 5–9 (2011). https://doi.org/10.1007/s13213-010-0077-5
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DOI: https://doi.org/10.1007/s13213-010-0077-5