A Simple Paper-Based α-Amylase Separating System for Potential Application in Biological Sciences

Abstract

The salivary glands are closely associated with stress conditions and the neuroendocrine system. The concentrations of these salivary biomarkers fluctuate with a person’s disease status. Until now, salivary diagnostics have been promising; however, the analytical tools for detecting salivary biomarkers have remained burdensome due to the lack of user-friendly and cost-effective equipment. In this study, we used a microfluidic starch-coated paper-based chip to isolate ɑ-amylase from human saliva. The concentration of ɑ-amylase on the head and tail sections of the paper-based chip was compared in the presence or absence of starch. We found that ɑ-amylase was highly concentrated only in the head section of the starch-coated paper-based chip through Western blotting (WB) and enzyme-linked immunosorbent assay (ELISA). We expect our designed paper-based chip will aid in α-amylase detection technologies using saliva and small amounts of other biofluids including blood, tears, pancreatic juice, and urine.

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Acknowledgements

This research was supported by Basic Science Research Program (2018R1A2B6005354) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT, Future Planning of Korea, and National Research Foundation of Korea (NRF-2018M3A9H1023323).

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Correspondence to Jong Wook Hong.

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Hyung, S., Karima, G., Shin, K. et al. A Simple Paper-Based α-Amylase Separating System for Potential Application in Biological Sciences. BioChip J (2021). https://doi.org/10.1007/s13206-021-00022-3

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Keywords

  • Paper-based chip
  • Saliva
  • α-Amylase
  • Microfluidics
  • Biomarkers
  • Chronic stress
  • Neuroendocrine system