Abstract
Visual volumetric chips based on gas-generating reactions can potentially be applied to instrument-free point-of-care testing (POCT). However, the structures of conventional chips and their detection methods are typically complicated due to two technical issues: (1) ink, as a distance marker, must be injected into a specified position before sample introduction, and (2) the gas-generating reaction must be initiated in a sealed state. In this study, an inkless volumetric chip operated with a micropipette was developed. The volumetric chip consisted of a reaction well (R well), a substrate well (S well) containing a H2O2 solution as an O2 generator, and a channel connecting the upper side of the R well and the lower side of the S well. Sample injection into the R well is followed by the insertion of a micropipette that is used to exert a negative pressure, which subsequently drives the transfer of a large portion of the H2O2 solution to the R well to initiate the gas-generating reaction. Meanwhile, a small portion of the H2O2 solution remains in the channel for use as a distance marker. The performance of the volumetric chip was evaluated by detecting Salmonella typhimurium, affording a limit of detection of 10 CFU within 20 min for culture samples and within 75 min for spiked milk samples. The volumetric chip developed herein is advantageous for POCT applications due to its simple structure and detection process.
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References
Engvall, E., Perlmann, P.: Enzyme-linked immunosorbent assay, ELISA. J. Immunol. 109, 129–135 (1972)
Lequin, R.M.: Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA). Clin. Chem. 51, 2415–2418 (2005)
Liu, D., Tian, T., Chen, X., Lei, Z., Song, Y., Shi, Y., Ji, T., Zhu, Z., Yang, L., Yang, C.: Gas-generating reactions for point-of-care testing. Analyst 143, 1294–1304 (2018)
Tian, T., Li, J., Song, Y., Zhou, L., Zhu, Z., Yang, C.J.: Distance-based microfluidic quantitative detection methods for point-of-care testing. Lab Chip 16, 1139–1151 (2016)
Li, Y., Uddayasankar, U., He, B., Wang, P., Qin, L.: Fast, sensitive, and quantitative point-of-care platform for the assessment of drugs of abuse in urine, serum, and whole blood. Anal. Chem. 89, 8273–8281 (2017)
Li, Y., Xuan, J., Song, Y., Wang, P., Qin, L.: A microfluidic platform with digital readout and ultra-low detection limit for quantitative point-of-care diagnostics. Lab Chip 15, 3300–3306 (2015)
Liu, D., Li, X., Zhou, J., Liu, S., Tian, T., Song, Y., Zhu, Z., Zhou, L., Ji, T., Yang, C.: A fully integrated distance readout ELISA-Chip for point-of-care testing with sample-in-answer-out capability. Biosens. Bioelectron. 96, 332–338 (2017)
Song, Y., Wang, Y., Qi, W., Li, Y., Xuan, J., Wang, P., Qin, L.: Integrative volumetric bar-chart chip for rapid and quantitative point-of-care detection of myocardial infarction biomarkers. Lab. Chip 16, 2955–2962 (2016)
Song, Y., Xia, X., Wu, X., Wang, P., Qin, L.: Integration of platinum nanoparticles with a volumetric bar-chart chip for biomarker assays. Angew. Chem. Int. Ed. 53, 12451–12455 (2014)
Song, Y., Zhang, Y., Bernard, P.E., Reuben, J.M., Ueno, N.T., Arlinghaus, R.B., Zu, Y., Qin, L.: Multiplexed volumetric bar-chart chip for point-of-care diagnostics. Nat. Commun. 3, 1283–1289 (2012)
Xie, Y., Wei, X., Yang, Q., Guan, Z., Liu, D., Liu, X., Zhou, L., Zhu, Z., Lin, Z., Yang, C.: A Shake&Read distance-based microfluidic chip as a portable quantitative readout device for highly sensitive point-of-care testing. Chem. Commun. 52, 13377–13380 (2016)
Lee, S., Kwon, D., Yim, C., Jeon, S.: Facile detection of troponin I using dendritic platinum nanoparticles and capillary tube indicators. Anal. Chem. 87, 5004–5008 (2015)
Wu, Z., Fu, Q., Yu, S., Sheng, L., Xu, M., Yao, C., Xiao, W., Li, X., Tang, Y.: Pt@AuNPs integrated quantitative capillary-based biosensors for point-of-care testing application. Biosens. Bioelectron. 85, 657–663 (2016)
Bu, S., Wang, K., Ju, C., Wang, C., Li, Z., Hao, Z., Shen, M., Wan, J.: Point-of-care assay to detect foodborne pathogenic bacteria using a low-cost disposable medical infusion extension line as readout and MnO2 nanoflowers. Food Control 98, 399–404 (2019)
Kwon, S., Choi, S.J.: Development of tubing-based stationary liquid-phase enzyme-linked immunosorbent assay. BioChip J. 13, 174–181 (2019)
Kim, M.-H., Choi, S.-J.: Immunoassay of paralytic shellfish toxins by moving magnetic particles in a stationary liquid-phase lab-on-a-chip. Biosens. Bioelectron. 66, 136–140 (2015)
Park, B., Choi, S.-J.: Sensitive immunoassay-based detection of Vibrio parahaemolyticus using capture and labeling particles in a stationary liquid phase lab-on-a-chip. Biosens. Bioelectron. 90, 269–275 (2017)
Iwase, T., Tajima, A., Sugimoto, S., Okuda, K., Hironaka, I., Kamata, Y., Takada, K., Mizunoe, Y.: A simple assay for measuring catalase activity: A visual approach. Sci. Rep. 3, 3081 (2013)
Hadwan, M.H., Ali, S.K.: New spectrophotometric assay for assessments of catalase activity in biological samples. Anal. Biochem. 542, 29–33 (2018)
Van Lente, F., Pepoy, M.: Coupled-enzyme determination of catalase activity in erythrocytes. Clin. Chem. 36, 1339–1343 (1990)
Shivakumar, A., Nagaraja, P., Chamaraja, N.A., Krishna, H., Avinash, K.: Determination of catalase activity using chromogenic probe involving iso-nicotinicacidhydrazide and pyrocatechol. J. Biotechnol. 155, 406–411 (2011)
Beers, R.F., Sizer, I.W.: A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J. Biol. Chem. 195, 133–140 (1952)
Mueller, S., Riedel, H.D., Stremmel, W.: Determination of catalase activity at physiological hydrogen peroxide concentrations. Anal. Biochem. 245, 55–60 (1997)
Wu, M., Lin, Z., Wolfbeis, O.S.: Determination of the activity of catalase using a europium(III)-tetracycline-derived fluorescent substrate. Anal. Biochem. 320, 129–135 (2003)
El Nashar, R.M.: Flow injection catalase activity measurement based on gold nanoparticles/carbon nanotubes modified glassy carbon electrode. Talanta 96, 161–167 (2012)
Acknowledgements
This work was supported by National Research Foundation of Korea grant funded by the Korean government (MSIT) [No. 2018R1A2B6005091] and by the 2019 Academic Research Support Program in Gangneung-Wonju National University.
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Han, H., Choi, SJ. Development of an Inkless, Visual Volumetric Chip Operated with a Micropipette. BioChip J 15, 179–186 (2021). https://doi.org/10.1007/s13206-021-00021-4
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DOI: https://doi.org/10.1007/s13206-021-00021-4