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Fluorometric Detection of Oncogenic EML4-ALK Fusion Gene based on a Graphene Oxide System

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Abstract

Detection of the fusion gene composed of echinoderm microtubule-associated protein-like 4 (EML4) and anaplastic lymphoma kinase (ALK) is important for diagnosis of lung adenocarcinoma. We developed a simple PCR-based fluorometric method for detecting the EML4-ALK fusion using a fluorescent probe DNA and graphene oxide (GO). The fluorophore-labeled probe DNA complementary to the EML4-ALK fusion junction was hydrolyzed during PCR by the 5′ to 3′ exonuclease activity of the Taq DNA polymerase. The fluorophore released from the probe DNA retained fluorescence without adsorption onto GO. Our GO-based fluorometric system was able to detect a single cancer cell harboring the EML4-ALK fusion in 106 wild-type cells.

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Acknowledgements

This research was supported by the National Research Foundation of Korea funded by the Ministry of Science, ICT & Future Planning (2017R1E1A1A01074656).

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Konkuk University, Gwangjin-gu, Seoul, 05029, Korea

    Yu Mi Baek, Yeeun Jeong & Dong-Eun Kim

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  1. Yu Mi Baek
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  2. Yeeun Jeong
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  3. Dong-Eun Kim
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Correspondence to Dong-Eun Kim.

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Conflict of Interests The authors declare no competing financial interests.

These authors contrilbuted equally.

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Baek, Y.M., Jeong, Y. & Kim, DE. Fluorometric Detection of Oncogenic EML4-ALK Fusion Gene based on a Graphene Oxide System. BioChip J 13, 370–377 (2019). https://doi.org/10.1007/s13206-019-3408-z

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