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Biologically Benign Multi-functional Mesoporous Silica Encapsulated Gold/Silver Nanorods for Anti-bacterial Applications by On-demand Release of Silver Ions

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Abstract

Although silver (Ag)-based nanoparticles (NPs) are frequently used for bactericidal purposes, they have critical issues including excessive release of Ag+, severe oxidation, and cytotoxicity. In this study, we designed a multifunctional, on-demand antibacterial agent by successively encapsulating bimetallic gold/silver nanorods (Ag/AgNRs) with mesoporous silica (mSiO2) shells. Au/AuNRs were synthesized by coating Ag on AuNRs in a controlled manner, so that they exhibited a localized surface plasmon resonance peak in the near-infrared (NIR) region. When Au/AgNR@mSiO2 NPs were irradiated with an NIR laser under optimal conditions (0.4 W/cm2), they generated a small amount of heat (40–45 oC), which successively triggered the release of Ag+ and induced bacterial cell death. Here, mSiO2 shells play critical roles because they not only protect Ag from oxidation but also prevent the burst release of Ag+ and improve biocompatibility of the antibacterial agent against normal cells. We found that this multifunctional bacterial agent effectively kills gram-negative Escherichia coli and gram-positive Staphylococcus aureus without significantly increasing the temperature of the medium. Au/AgNR@mSiO2 NPs were also biologically benign with high biocompatibility against mammalian cells.

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References

  1. 1.

    Unser, S., Bruzas, I., He, J. & Sagle, L. Localized Surface Plasmon Resonance Biosensing: Current Challenges and Approaches. Sensors15, 15684–15716 (2015).

  2. 2.

    Ayala-orozco, C., Urban, C., Knight, M.W., Urban, A.S., Neumann, O., Bishnoi, S.W., Mukherjee, S., Goodman, A.M., Charron, H., Mitchell, T., Shea, M., Roy, R., Nanda, S., Schiff, R., Halas, N.J. & Joshi A. Au Nanomatryoshkas as Efficient Near-infrared Photothermal Transducers for Cancer Treatment?: Benchmarking against Nanoshells. ACS Nano6, 6372–6381 (2014).

  3. 3.

    Lin, L.S., Yang, X., Zhou, Z., Yang, Z., Jacobson, O., Liu, Y., Yang, A., Niu, G., Song, J., Yang, H.H & Chen, X. Yolk - Shell Nanostructure: An Ideal Architecture to Achieve Harmonious Integration of Magnetic - Plasmonic Hybrid Theranostic Platform. Adv. Mater.29, 1606681 (2017).

  4. 4.

    Javid, A., Kumar, M., Yoon, S., Lee, J.H. & Han, J.G. Size-Controlled Growth and Antibacterial Me chanism for Cu:C Nanocomposite Thin Films. Phys. Chem. Chem. Phys.19, 237–244 (2017).

  5. 5.

    Tan, L.H., Xing, H., Chen, H. & Lu, Y. Facile and Efficient Preparation of Anisotropic DNA-Functionalized Gold Nanoparticles and Their Regioselective Assembly. J. Am. Chem. Soc.135, 17675–17678 (2013).

  6. 6.

    Chaudhuri, R.G. & Paria, S. Core / Shell Nanoparticles?: Classes, Properties, Synthesis Mechanisms, Characterization, and Applications. Chem. Rev.112, 2373–2433 (2012).

  7. 7.

    Noh, J.Y., Kim, J.I., Chang, Y.W., Park, J.M., Song, H.W., Kang, M.J. & Pyun, J.C. Gold Nanoislands Chip for Laser Desorption/Ionization (LDI) Mass Spectrometry. BioChip J.11, 246–254 (2017).

  8. 8.

    Liang, H., Liu, B., Yuan, Q. & Liu, J. Magnetic Iron Oxide Nanoparticle Seeded Growth of Nucleotide Coordinated Polymers. ACS Appl. Mater.8, 15615–15622 (2016).

  9. 9.

    Lee, J.W., Jung, H., Cho, H.H., Lee, J.H. & Nam, Y. Gold Nanostar-Mediated Neural Activity Control using Plasmonic Photothermal Effects. Biomaterials153, 59–69 (2018).

  10. 10.

    Kim, B.H., Yoon, I.S. & Lee, J.-S. Masking Nano-particle Surfaces for Sensitive and Selective Colorimetric Detection of Proteins. Anal. Chem.85, 10542–10548 (2013).

  11. 11.

    Willets, K.A. & Van Duyne, R.P. Localized Surface Plasmon Resonance Spectroscopy and Sensing. Annu. Rev. Phys. Chem.58, 267–297 (2007).

  12. 12.

    Loo, C., Lin, A., Hirsch, L., Lee, M.-H., Barton, J., Halas, N., West, J. & Drezek, R. Nanoshell-Enabled Photonics-Based Imaging and Therapy of Cancer. Technol. Cancer Res. Treat.3, 33–40 (2004).

  13. 13.

    Liang, X., Govindaraju, S. & Yun, K. Dual Applicability of Polyaniline Coated Gold Nanorods: a Study of Antibacterial and Redox Activity. BioChip J.12, 137–145 (2018).

  14. 14.

    Yoon, S., Lee, B., Yun, J., Han, J.G., Lee, J.S. & Lee, J.H. Systematic Study of Interdependent Relationship on Gold Nanorod Synthesis Assisted by Electron Microscopy Image Analysis. Nanoscale9, 7114–7123 (2017).

  15. 15.

    Park, C., Kong, M., Lee, J.-H., Ryu, S. & Park, S. Detection of Bacillus Cereus Using Bioluminescence Assay with Cell Wall-Binding Domain Conjugated Magnetic Nanoparticles. BioChip J.12, 287–293 (2018).

  16. 16.

    Ding, X., Yuan, P., Gao, N., Zhu, H., Yang, Y.Y. & Xu, Q.H. Au-Ag Core-Shell Nanoparticles for Simultaneous Bacterial Imaging and Synergistic Antibacterial Activity. Nanomed. Nanotechnol. Biol. Med.13, 297–305 (2017).

  17. 17.

    Dos Santos, C.A., Seckler, M.M., Ingle, A.P., Gupta, I., Galdiero, S., Galdiero, M., Gade, A. & Rai, M. Silver Nanoparticles?: Therapeutical Uses, Toxicity, and Safety Issues. J. Pharm. Sci. 1931–1944 (2014).

  18. 18.

    Jung, W.K., Koo, H.C., Kim, K.W., Shin, S., Kim, S.H. & Park, Y.H. Antibacterial Activity and Mechanism of Action of the Silver Ion in Staphylococcus Aureus and Escherichia Coli. Appl. Environ. Microbiol.74, 2171–2178 (2008).

  19. 19.

    Black, K.C.L., Sileika, T.S., Yi, J., Zhang, R., Rivera, J.G. & Messersmith, P.B. Bacterial Killing by Light-Triggered Release of Silver from Biomimetic Metal Nanorods. Small10, 169–178 (2014).

  20. 20.

    Jiang, X., Wang, L., Ji, Y., Tang, J., Tian, X., Cao, M., Li, J., Bi, S., Wu, X., Chen, C. & Yin, J.-J. Interference of Steroidogenesis by Gold Nanorod Core/Silver Shell Nanostructures: Implications for Reproductive Toxicity of Silver Nanomaterials. Small13, 1602855 (2017).

  21. 21.

    Black, Burd, A., Kwok, C. H., Hung, S. C., Chan, H. S., Gu, H., Lam, W. K., Huang, L. A Comparative Study of the Cytotoxicity of Silver-Based Dressings in Monolayer Cell, Tissue Explant, and Animal Models. Wound Rep. Reg.15, 94–104 (2007).

  22. 22.

    Zhang, Z., Wang, L., Wang, J., Jiang, X., Li, X., Hu, Z., Ji, Y., Wu, X. & Chen, C. Mesoporous Silica-Coated Gold Nanorods as a Light-Mediated Multifunctional Theranostic Platform for Cancer Treatment. Adv. Mater.24, 1418–1423 (2012).

  23. 23.

    Yoon, S., Lee, B., Kim, C. & Lee, J.H. Controlled Heterogeneous Nucleation for Synthesis of Uniform Mesoporous Silica-Coated Gold Nanorods with Tailorable Rotational Diffusion and 1 nm-Scale Size Tunability. Cryst. Growth Des.18, 4731–4736 (2018).

  24. 24.

    Lee, J.-H., Kang, S., Ahn, M., Jang, H. & Min, D.H. Development of Dual-Pore Coexisting Branched Silica Nanoparticles for Efficient Gene-Chemo Cancer Therapy. Small14, 1702564 (2018).

  25. 25.

    Cheng, W., Liang, C., Xu, L., Liu, G., Gao, N., Tao, W., Luo, L., Zuo, Y., Wang, X., Zhang, X., Zeng, X. & Mei, L. TPGS-Functionalized Polydopamine-Modified Mesoporous Silica as Drug Nanocarriers for Enhanced Lung Cancer Chemotherapy Against Multidrug Resistance. Small13, 1700623 (2017).

  26. 26.

    Kwon, D., Cha, B.G., Cho, Y., Min, J., Park, E.B., Kang, S.J. & Kim, J. Extra-Large Pore Mesoporous Silica Nanoparticles for Directing in Vivo M2 Macrophage Polarization by Delivering IL-4. Nano Lett.17, 2747–2756 (2017).

  27. 27.

    Song, T., Tang, L., Tan, L.H., Wang, X., Satyavolu, N.S.R., Xing, H., Wang, Z., Li, J., Liang, H. & Lu, Y. DNA-Encoded Tuning of Geometric and Plasmonic Properties of Nanoparticles Growing From Gold Nanorod Seeds. Angew. Chem. Int. Ed.54, 8114–8118 (2015).

  28. 28.

    Kim, F., Song, J.H. & Yang, P. Photochemical Synthesis of Gold Nanorods. J. Am. Chem. Soc.124, 14316–14317 (2002).

  29. 29.

    Heo, J.H., Yi, G.S., Lee, B.S., Cho, H.H., Lee, J.W. & Lee, J.H. A Significant Enhancement of Color Transition From an on - off Type Achromatic Colorimetric Nanosensor for Highly Sensitive Multi-Analte Detection with the Naked Eye. Nanoscale8, 18341–18351 (2016).

  30. 30.

    Liu, X., Wang, Q., Li, C., Zou, R., Li, B., Song, G., Xu, K., Zheng, Y. & Hu, J. Cu2-xSe@mSiO2-PEG Core-Shell Nanoparticles: A Low-Toxic and Efficient Difunctional Nanoplatform for Chemo-Photothermal Therapy under Near Infrared Light Radiation with a Safe Power Density. Nanoscale.6, 4361–4370 (2014).

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Acknowledgements

This research was supported by the National Research Foundation (NRF) of Korea for a Bio-inspired Innovation Technology Development Project (NRF-2018M3C1B7021997) funded by the Ministry of Science and ICT and Basic Science Research Program funded by the Ministry of Education (NRF-2019R1A6A1A03033215). This work was also supported by Basic Science Research Capacity Enhancement Project through Korea Basic Science Institute (National Research Facilities and Equipment Center) grant funded by the Ministry of Education (2019R1A6C1010031).

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Correspondence to Jung Heon Lee.

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Conflict of Interests The authors declare no competing financial interests.

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Yoon, S., Chung, Y., Lee, J.W. et al. Biologically Benign Multi-functional Mesoporous Silica Encapsulated Gold/Silver Nanorods for Anti-bacterial Applications by On-demand Release of Silver Ions. BioChip J 13, 362–369 (2019). https://doi.org/10.1007/s13206-019-3407-0

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Keywords

  • Gold/Silver nanorod
  • Mesoporous silica
  • Antibacterial agent
  • Drug delivery
  • Near-infrared (NIR)
  • Controlled silver release
  • Biocompatibility