Abstract
Sulfamethazine (SMZ) belonging to sulfonamide antibiotics is an important surveillance target because of its widespread presence in food materials and processed foods. In this study, a new detection on SMZ was conducted with an indirect-competitive optical waveguide lightmode spectroscopy-based immunosensor. As sensing element, the anti-SMZ antibody had good reactivity and specificity to SMZ-BSA as coating antigen. The sensor signals during the whole course of reaction comprising immobilization of the coating antigen and competitive immune reaction were stable enough to acquire reproducible results. The concentrations of the coating antigen and antibody selected for the immunosensing were 2 and 0.03129 mg/mL, respectively. When determined with 10-12-10-2 M SMZ, the limit of detection of the present sensor was presumed to be 1 pM. The coefficient of variability for five repetitive measurements using the same sensor chip was 5.41%. These results seemed to indicate that the immunosensor has a good potential to be used as a high-sensitivity analytical tool for SMZ in food matrices.
Similar content being viewed by others
References
Saschenbrecher, P.W. & Fish, N.A. Sulphamethazine residues in uncooked edible tissues of pork following recommended oral administration and withdrawal. Can. J. Compar. Med. 44, 338–345 (1980).
Wen, Y., Zhang, M., Zhao, Q. & Feng, Y.Q. Monitoring of five sulfonamides antibacterial residues in milk by in-tube solid-phase microextraction coupled to highperformance liquid chromatography. J. Agr. Food Chem. 53, 8468–8473 (2005).
Wang, L., Wang, S., Zhang, J., Liu, J. & Zhang, Y. Enzyme-linked immunosorbent assay and colloidal gold immunoassay for sulphamethazine residues in edible animal foods: investigation of the effects of the analytical conditions and the sample matrix on assay performance. Anal. Bioanal. Chem. 390, 1619–1627 (2008).
Situ, C., Crooks, S.R.H., Baxter, A.G., Ferguson, J. & Elliott, C.T. On-line detection of sulfamethazine and sulfadiazine in porcine bile using a multi-channel high-throughput SPR biosensor. Anal. Chim. Acta 473, 143–149 (2002).
Huertas-Pérez, J.F., Arroyo-Manzanares, N., Havlíková, L., Gámiz-Gracia, L., Solich, P. & García-Campaña, A.M. Method optimization and validation for the determination of eight sulfonamides in chicken muscle and eggs by modified QuEChERS and liquid chromatography with fluorescence detection. J. Pharm. Biomed. Anal. 124, 261–266 (2016).
Li, H. & Kijak, P.J. Development of a quantitative multiclass/multiresidue method for 21 veterinary drugs in shrimp. J. AOAC Int. 94, 394–406 (2011).
Xu, W., Su, S., Jiang, P., Wang, H., Dong, X. & Zhang, M. Determination of sulfonamides in bovine milk with column-switching high performance liquid chromatography using imprinted silica with hydrophilic external layer as restricted access and selective extraction material. J. Chromatogr. A 1217, 7198–7207 (2010).
Hu, L., Zuo, P. & Ye, B.C. Multicomponent mesofluidic system for the detection of veterinary drug residues based on competitive immunoassay. Anal. Biochem. 405, 89–95 (2010).
Jiménez, V., Adrian, J., Guiteras, J., Marco, M.P. & Companyó, R. Validation of an enzyme-linked immunosorbent assay for detecting sulfonamides in feed resources. J. Agr. Food Chem. 58, 7525–7531 (2010).
Reguera, C., Ortiz, M.C., Herrero, A. & Sarabia, L.A. Optimization of a FIA system with amperometric detection by means of a desirability function: determination of sulfadiazine, sulfamethazine and sulfamerazine in milk. Talanta 75, 274–283 (2008).
Rebe Raz, S., Bremer, M.G., Haasnoot, W. & Norde, W. Label-free and multiplex detection of antibiotic residues in milk using imaging surface plasmon resonancebased immunosensor. Anal. Chem. 81, 7743–7749 (2009).
Sternesjö, A., Mellgren, C. & Björck, L. Determination of sulfamethazine residues in milk by a surface plasmon resonance-based biosensor assay. Anal. Biochem. 226, 175–182 (1995).
Kim, N., Kim, D.-K., Cho, Y.-J., Moon, D.-K. & Kim, W.-Y. Carp vitellogenin detection by an optical waveguide lightmode spectroscopy biosensor. Biosens. Bioelectron. 24, 391–396 (2008).
Diamandis, E.P. & Christopoulos, T.K. Europium chelate labels in time-resolved fluorescence immunoassays and DNA hybridization assays. Anal. Chem. 62, 1149A–1157A (1990).
Handl, H.L. & Gillies, R.J. Lanthanide-based luminescent assays for ligand-receptor interactions. Life Sci. 77, 361–371 (2005).
Park, I.-S. & Kim, N. Development of a chemiluminescent immunosensor for chloramphenicol. Anal. Chim. Acta 578, 19–24 (2006).
Oh, B.-K., Kim, Y.-K., Park, K.W., Lee, W.H. & Choi, J.-W. Surface plasmon resonance immunosensor for the detection of Salmonella typhimurium. Biosens. Bioelectron. 19, 1497–1504 (2004).
Kim, N., Park, I.-S. & Kim, D.-K. Characteristics of a label-free piezoelectric immunosensor detecting Pseudomonas aeruginosa. Sensor Actuat. B-Chem. 100, 432–438 (2004).
Vörös, J. et al. Optical grating coupler biosensors. Biomaterials 23, 3699–3710 (2002).
Benesch, J., Askendl, A. & Tengvall, P. Quantification of adsorbed human serum albumin at solid interfaces: A comparison between radioimmunoassay (RIA) and simple null ellipsometry. Colloid Surface B-Biointer. 18, 71–81 (2000).
Huetz, P. et al. Reactivities of antibodies on antigens adsorbed on solid surfaces. Proteins Interfaces II 602, 334–349 (1995).
Homola, J., Yee, S.S. & Gauglitz, G. Surface plasmon resonance sensors: review. Sensor Actuat. B-Chem. 54, 3–15 (1999).
Kim, N., Kim, D.-K. & Kim, W.-Y. Sulfamethazine detection with direct-binding optical waveguide lightmode spectroscopy-based immunosensor. Food Chem. 108, 768–773 (2008).
Ma, M. et al. Chemiluminescence resonance energy transfer competitive immunoassay employing haptenfunctionalized quantum dots for the detection of sulfamethazine. ACS Appl. Mater. Inter. 8, 11745–11750 (2016).
Moon, D.-K., Kim, N. & Kim, W.-Y. Reactivity of the antibodies against purified carp vitellogenin and a synthetic vitellogenin peptide. J. Korean Soc. Appl. Biol. Chem. 49, 196–201 (2006).
Szalontai, H., Adányi, N. & Kiss, A. Comparative determination of two probiotics by QCM and OWLSbased immunosensors. New Biotechnol. 31, 395–401 (2014).
Clerc, D. & Lukosz, W. Direct immunosensing with an integrated-optical output grating coupler. Sensor Actuat. B-Chem. 40, 53–58 (1997).
Majer-Baranyi, K. et al. Optical waveguide lightmode spectroscopy technique-based immunosensor development for aflatoxin B1 determination in spice paprika samples. Food Chem. 211, 972–977 (2016).
Kim, J., Jeon, M., Paeng, K.J. & Paeng, I.R. Competitive enzyme-linked immunosorbent assay for the determination of catecholamine, dopamine in serum. Anal. Chim. Acta 619, 87–93 (2008).
Kim, N., Park, I.-S. & Kim, W.-Y. Salmonella detection with a direct-binding optical grating coupler immunosensor. Sensor Actuat. B-Chem. 121, 606–615 (2007).
Kim, N., Kim, D.-K. & Cho, Y.-J. Development of indirect-competitive quartz crystal microbalance immunosensor for C-reactive protein. Sensor Actuat. B-Chem. 143, 444–448 (2009).
Hálamek, J., Makower, A., Skládal, P. & Scheller, F.W. Highly sensitive detection of cocaine using piezoelectric immunosensor. Biosens. Bioelectron. 17, 1045–1050 (2002).
Shitandi, A., Oketch, A. & Mahungum, S. Evaluation of a Bacillus stearothermophilus tube test as a screening tool for anticoccidial residues in poultry. J. Vet. Sci. 7, 177–180 (2006).
Vallejo, R.P., Bogus, E.R. & Mumma, R.O. Effects of hapten structure and bridging groups on antisera specificity in parathion immunoassay development. J. Agr. Food Chem. 30, 572–580 (1982).
Ryu, H.-S., Jang, Y.-I. & Kim, W.-Y. Immunoblotting reactivity of vitellogenin antibodies against native vitellogenins and a vitellogenin protein fragment produced in E. coli. J. Appl. Biol. Chem. 52, 170–173 (2009).
Bovaird, J.H., Ngo, T.T. & Lenhoff, H.M. Optimizing the o-phenylenediamine assay for horseradish peroxidase: Effects of phosphate and pH, substrate and enzyme concentrations, and stopping reagents. Clin. Chem. 28, 2423–2426 (1982).
Polzius, R., Schneider, Th., Bier, F.F., Bilitewski, U. & Koschinski, W. Optimization of biosensing using grating couplers: immobilization on tantalum oxide waveguides. Biosens. Bioelectron. 11, 503–514 (1996).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kim, N. Development of Indirect-Competitive Optical Waveguide Lightmode Spectroscopy-based Immunosensor for Measuring Sulfamethazine. BioChip J 12, 128–136 (2018). https://doi.org/10.1007/s13206-017-2205-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13206-017-2205-9