Various secondary metabolites derived from plants are useful for humans to preserve their health. For this reason, medical herbs have been consumed universally without deep research about the dangerousness of these substance. However, they can produce unexpected adverse reactions in humans. Among these side effects, allergic reactions involving human immunoglobulin E can occur due to antimicrobial or antibacterial substances in plants. Glycyrrhiza uralensis has been prescribed for the treatment of numerous diseases given its multiple types of pharmaceutical efficacy, including its antimicrobial and antibacterial activities. Therefore, we researched whether Glycyrrhiza uralensis, which has antimicrobial ability, can cause allergic reaction in humans. To test the hypothesis devised here, we conducted proteomic level experiments. We utilized SDS-PAGE using a protein extract of Glycyrrhiza uralensis, with Coomassie blue staining to confirm the protein pattern and western blotting to identify Human IgE binding proteins in Glycyrrhiza uralensis. Among the many candidate sera, these methods detected three positive sera. Moreover, an identified protein section with reactivity associated with Human IgE was analyzed by LC-MS/ MS. Our experimental data provide a basis for testing the possibility of Glycyrrhiza uralensis as an allergen by identifying the reactivity between human Immunoglobulin E and proteins of Glycyrrhiza uralensis.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Sampedro, J. & Valdivia, E.R. New Antimicrobial Agents of Plant Origin. in Antimicrobial Compounds: Current Strategies and New Alternatives (eds. Villa, T.G. & Veiga-Crespo, P.) 83–114 (Springer Berlin Heidelberg, Berlin, Heidelberg, 2014).
Park, J. & Jang, H.-J. Anti-diabetic effects of natural products an overview of therapeutic strategies. Mol. Cell. Toxicol. 13, 1–20 (2017).
Jang, S.-A. et al. Gamma irradiation-induced liver injury and its amelioration by red ginseng extract. Mol. Cell. Toxicol. 13, 461–469 (2017).
Nilnumkhum, A., Punvittayagul, C., Chariyakornkul, A. & Wongpoomchai, R. Effects of hydrophilic compounds in purple rice husk on AFB1-induced mutagenesis. Mol. Cell. Toxicol. 13, 171–178 (2017).
Cowan, M.M. Plant Products as Antimicrobial Agents. Clin. Microbiol. Rev. 12, 564–582 (1999).
Phannasorn, W., Khanaree, C., Wongnoppavich, A. & Chewonarin, T. The effect of purple rice (Oryza sativa L. indica) extract on the inflammatory response in a colon cancer cell line and dextran sulfate-induced tumor promotion in the rat colon. Mol. Cell. Toxicol. 13, 433–442 (2017).
Middleton, E., Jr., Kandaswami, C. & Theoharides, T.C. The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacol. rev. 52, 673–751 (2000).
Cunha, B.A. Antibiotic Side Effects. Med. Clin. North Am. 85, 149–185 (2001).
Kokoska, L., Polesny, Z., Rada, V., Nepovim, A. & Vanek, T. Screening of some Siberian medicinal plants for antimicrobial activity. J. Ethnopharmacol. 82, 51–53 (2002).
Hoffmann-Sommergruber, K. Plant Allergens and Pathogenesis-Related Proteins. Int. arch. allergy immunol. 122, 155–166 (2000).
Sampson, H.A. Food anaphylaxis. BMJ 56, 925–935 (2000).
Asl, M.N. & Hosseinzadeh, H. Review of Pharmacological Effects of Glycyrrhiza sp. and its Bioactive Compounds. Phytother. Res. 22, 709–724 (2008).
Gafner, S. et al. Isoflavonoids and Coumarins from Glycyrrhiza uralensis: Antibacterial Activity against Oral Pathogens and Conversion of Isoflavans into Isoflavan-Quinones during Purification. J. nat. prod. 74, 2514–2519 (2011).
Zhao, Z., Wang, W., Guo, H. & Zhou, D. Antidepressant-like effect of liquiritin from Glycyrrhiza uralensis in chronic variable stress induced depression model rats. Behavioural Brain Research 194, 108–113 (2008).
Gruchalla, R.S. & Pirmohamed, M. Antibiotic Allergy. N. Engl. J. Med. 354, 601–609 (2006).
Kim, K.-H. et al. Isolated protein of Astragalus membranaceus acts as an allergen by binding human immunoglobulin E on human sera. BioChip J. 10, 95–102 (2016).
Kim, K.-H., Park, J.Y., Lee, I.-S., Kim, Y. & Jang, H.-J. Proteins derived from Prunus armeniaca kernel are possible to cause Immunoglobulin E reactivity in human sera. Mol. Cell. Toxicol. 13, 213–220 (2017).
Li, S., Geng, F., Wang, P., Lu, J. & Ma, M. Proteome analysis of the almond kernel (Prunus dulcis). J. sci. food agric. 96, 3351–3357 (2016).
Al-Ghouleh, A. et al. The glycosylation pattern of common allergens: the recognition and uptake of Der p 1 by epithelial and dendritic cells is carbohydrate dependent. PLoS One 7, e33929 (2012).
Nierman, W.C. et al. Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus. Nature 438, 1151–1156 (2005).
Bishop, J.G., Dean, A.M. & Mitchell-Olds, T. Rapid evolution in plant chitinases: molecular targets of selection in plant-pathogen coevolution. Proc Natl Acad Sci U S A 97, 5322–5327 (2000).
Yagami, T. [Plant defense-related proteins as latex allergens]. Kokuritsu Iyakuhin Shokuhin Eisei Kenkyujo hokoku=Bulletin of National Institute of Health Sciences, 46–62 (1998).
Yagami, T., Sato, M., Nakamura, A. & Shono, M. One of the rubber latex allergens is a lysozyme. J. allergy clin. immunol. 96, 677–686 (1995).
Proteau, P.J. 1-Deoxy-d-xylulose 5-phosphate reductoisomerase: an overview. Bioorg. Chem. 32, 483–493 (2004).
Takahashi, S., Kuzuyama, T., Watanabe, H. & Seto, H. A 1-deoxy-d-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-d-erythritol 4-phosphate in an alternative nonmevalonate pathway for terpenoid biosynthesis. Proc. Natl. Acad. Sci. 95, 9879–9884 (1998).
Bailey, C.J. & Day, C. Traditional Plant Medicines as Treatments for Diabetes. Diabetes Care 12, 553–564 (1989).
van Vuurden, D.G. et al. PARP inhibition sensitizes childhood high grade glioma, medulloblastoma and ependymoma to radiation. Oncotarget 2, 984–996 (2011).
Kim, K.-W. et al. Osteogenic differentiation of human mesenchymal stem cells promoted by the crude extracts of the mixture of Cortex mori radicis, Patrinia saniculaefolia. mol. Cell. Toxicol. 11, 475–482 (2015).
Lee, S.Y. et al. Heterogeneous expression of Chla mydia pneumoniae antigen candidates and high-level soluble expression of its inclusion membrane proteins in Escherichia coli. Mol. Cell. Toxicol. 13, 387–394 (2017).
Kang, H., Park, B.-R., Yoo, H.-S., Kwon, K.-R. & Kang, I.-C. Anti-angiogenic function of a Korean Ginseng and Toad venom complex, Doksamsumsu-dan (DSSSD) analyzed by a forwarded phase antibody microarray. BioChip J. 9, 222–231 (2015).
Bahk, Y.Y. et al. Antigens secreted from Mycobacterium tuberculosis: identification by proteomics approach and test for diagnostic marker. Proteomics 4, 3299–3307 (2004).
Gobom, J., Nordhoff, E., Mirgorodskaya, E., Ekman, R. & Roepstorff, P. Sample purification and preparation technique based on nano-scale reversed-phase columns for the sensitive analysis of complex peptide mixtures by matrix-assisted laser desorption/ionization mass spectrometry. J. mass spectrom. 34, 105–116 (1999).
Byeon, J., Kang, K.H., Jung, H.-K. & Suh, J.-K. Assessment for quantification of biopharmaceutical protein using a microvolume spectrometer on microfluidic slides. BioChip J. 11, 21–29 (2017).
About this article
Cite this article
An, EJ., Kim, KH., Lee, IS. et al. Identification of Possibility of Glycyrrhiza uralensis as an Allergen by Protein Analysis. BioChip J 12, 75–82 (2018). https://doi.org/10.1007/s13206-017-2110-2
- herbal medicine
- plant protein