Development of a Sol-gel-assisted Reverse-phase Microarray Platform for Simple and Rapid Detection of Prostate-specific Antigen from Serum

Abstract

A sol-gel-based reverse-phase microarray was developed with improved sensitivity for prostatespecific antigen (PSA) from serum. The pore-sizecontrolled 3D sol-gel matrix was created with a large surface area to capture target molecules densely. Using the optically active sol-gel nanocomposites, human female serum was spiked with PSA and assessed using the reverse-phase protein microarray. The reverse-phase assay exhibited a limit of detection of 1 pg/mL for PSA and a dynamic range of 103 orders of magnitude. Notably, the platform matched the demand of the immunoassay in a simple and feasible manner. Moreover, the platform shortened the total assay time with an increased accuracy of diagnosis.

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Correspondence to Ok Chan Jeong or Soyoun Kim.

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Lee, S., Laurell, T., Jeong, O.C. et al. Development of a Sol-gel-assisted Reverse-phase Microarray Platform for Simple and Rapid Detection of Prostate-specific Antigen from Serum. BioChip J 12, 69–74 (2018). https://doi.org/10.1007/s13206-017-2109-8

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Keywords

  • Sol-gel microarray
  • Macro/nano-structure silicon
  • Reverse-phase assay
  • Prostate-specific antigen
  • Prostate cancer