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Label-free Microarray-based Binding Affinity Constant Measurement with Modified Fluidic Arrangement

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Abstract

The oblique-incidence reflectivity difference (OI-RD) scanning microscopy has the capability of simultaneously measuring binding curves of a protein probe with tens of thousands molecular targets in a microarray and yielding reaction rate constants. However, the quality of reaction rate constants is influenced by the fluidic system. To improve the quality of reaction rate constant measurement over the entire microarray, we demonstrate a fluidic chamber that allows the fluid to flow from the bottom to the top uniformly across the microarray and thus provides more uniform and accurate measurements of reaction rate constants with simplified fluidic design.

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou, 221116, China

    Junwei Hu, Caiqin Han & Hao Chen

  2. Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-Precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Fudan University, Shanghai, 200433, China

    Ru Chen, Chenggang Zhu, Bilin Ge, Lan Mi, Jiong Ma & Yiyan Fei

  3. Department of Physics, University of California, Davis, California, 95616, USA

    Xiangdong Zhu

Authors
  1. Junwei Hu
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  2. Ru Chen
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  3. Chenggang Zhu
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  4. Bilin Ge
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  5. Xiangdong Zhu
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  6. Lan Mi
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  7. Jiong Ma
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  8. Caiqin Han
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  9. Hao Chen
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  10. Yiyan Fei
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Corresponding authors

Correspondence to Hao Chen or Yiyan Fei.

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Hu, J., Chen, R., Zhu, C. et al. Label-free Microarray-based Binding Affinity Constant Measurement with Modified Fluidic Arrangement. BioChip J 12, 11–17 (2018). https://doi.org/10.1007/s13206-017-2102-2

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  • DOI: https://doi.org/10.1007/s13206-017-2102-2

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