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Colorimetric Schirmer strip for tear glucose detection

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Abstract

Human tears have attracted attention with easy-accessible indicators of healthcare monitoring. Recently self-monitoring of tear glucose in place of blood glucose has been developed with microfluidic paper-based analysis for a non-invasive diagnosis of diabetes. However, most previous works still have many limitations in unstable sample collection, low sensitivity, and insufficient sample volume. Here, we report a colorimetric Schirmer strip with preconcentration for tear glucose detection. A wax barrier on a paper strip enables tear collection of a fixed quantity as well as biocompatible incorporation of tear collection and detection by preventing flow reversal of reagents. Additional solvent was injected on a sampling site after tear collection, which accumulates the dispersed glucose into active site by capillary-driven flow for signal amplification. Glucose concentration was quantitatively detected at 0.1-2 mM by using a simple colorimetric method without any further image processing. The color changes in glucose concentration were clearly distinguishable between normal and diabetic patient-level. The colorimetric paper strip allows straightforward sensing platform for noninvasive diagnosis of diabetes and direct application of paper-based technology to the human body fluids.

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Authors and Affiliations

  1. Department of Bio and Brain Engineering and KAIST Institute for Optical Science and Technology, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea

    Byoung-Hoon Kang, Moonseong Park & Ki-Hun Jeong

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  1. Byoung-Hoon Kang
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  2. Moonseong Park
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  3. Ki-Hun Jeong
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Correspondence to Ki-Hun Jeong.

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Kang, BH., Park, M. & Jeong, KH. Colorimetric Schirmer strip for tear glucose detection. BioChip J 11, 294–299 (2017). https://doi.org/10.1007/s13206-017-1405-7

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  • DOI: https://doi.org/10.1007/s13206-017-1405-7

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