Abstract
Microalbuminuria is a key indicator for the treatment of cardiovascular or cerebrovascular diseases and death. Therefore, sensitive and specific detection methods of HSA in biological fluids especially in low concentration are necessary in diagnosis and preventive medicine. Here, nanostructured electrical immunosensor is demonstrated for the rapid, sensitive and selective detection of human serum albumin (HSA), using single-walled carbon nanotube (swCNT) field-effect transistor (FETs) functionalized with monoclonal anti-human serum albumin (mAHSA) via simple coating. The selective binding of HSA with mAHSA on the surface of swCNT induces the electrostatic gating effect to the swCNT-FET. The resulting sensor exhibited high sensitivity toward HSA in real time, with good selectivity. The proposed method provides a powerful platform for a real time, sensitive and selective monitoring of HSA in biological fluids.
Similar content being viewed by others
References
Moina, C. & Ybarra, G. Fundamentals and Applications of Immunosensors, Advances in Immunoassay Technology. Dr. Norman H.L. Chiu (Ed.), InTech (2012).
Ricci, F., Volpe, G., Micheli, L. & Palleschi, G. A review on novel developments and applications of immunosensors in food analysis. Anal. Chim. Acta 605, 111–129 (2007).
Lim, S.A. & Ahmed, M.U. Electrochemical immunosensors and their recent nanomaterial-based signal amplification strategies: a review. RSC Adv. 6, 24995–25014 (2016).
Li, M. et al. Three-dimensional hierarchical plasmonic nano-architecture enhanced surface-enhanced Raman scattering immunosensor for cancer biomarker detection in blood plasma. Acs Nano 7, 4967–4976 (2013).
Pei, X. et al. Sandwich-type immunosensors and immunoassays exploiting nanostructure labels: A review. Anal. Chim. Acta 758, 1–18 (2013).
Kim, T.H. et al. “Bioelectronic super-taster” device based on taste receptor-carbon nanotube hybrid structures. Lab Chip 11, 2262–2267 (2011).
Kim, T.H. et al. Selective and sensitive TNT sensors using biomimetic polydiacetylene-coated CNT-FETs. ACS Nano 5, 2824–2830 (2011).
Kim, T.H. et al. Single-carbon-atomic-resolution detection of odorant molecules using a human olfactory receptor-based bioelectronic nose. Adv. Mater. 21, 91–94 (2009).
Kang, J. et al. Large-scale assembly of carbon nanotube-based flexible circuits for DNA sensors. Nanotechnology 19, 135305 (2008).
Peters, T. All about albumin: biochemistry, genetics, and medical applications. Academic Press, San Diego (1996).
Smales, C.M. & James, D.C. Therapeutic proteins: methods and protocols. Humana Press, Totowa, N.J. (2005).
Wu, H.Y. et al. Diagnostic performance of random urine samples using albumin concentration vs ratio of albumin to creatinine for microalbuminuria screening in patients with diabetes mellitus: a systematic review and meta-analysis. JAMA Intern. Med. 174, 1108–1115 (2014).
Fanali, G. et al. Human serum albumin: From bench to bedside. Mol. Aspects Med. 33, 209–290 (2012).
Alekseev, R.J. & Rebane, A.L. Serum albumin: structure, functions, and health impact. Nova Science Publishers, New York (2012).
Park, H.Y., Schumock, G.T., Pickard, A.S. & Akhras, K. A structured review of the relationship between microalbuminuria and cardiovascular events in patients with diabetes mellitus and hypertension. Pharmacotherapy 23, 1611–1616 (2003).
Caballero, D., Martinez, E., Bausells, J., Errachid, A. & Samitier, J. Impedimetric immunosensor for human serum albumin detection on a direct aldehyde-functionalized silicon nitride surface. Anal. Chim. Acta 720, 43–48 (2012).
Niwa, T. et al. Enzyme immunoassay for urinary albumin at low concentration in diabetes mellitus. Clin. Chim. Acta 186, 391–396 (1990).
Fielding, B.A., Price, D.A. & Houlton, C.A. Enzyme immunoassay for urinary albumin. Clin. Chem. 29, 355–357 (1983).
Langer, K. et al. Optimization of the preparation process for human serum albumin (HSA) nanoparticles. Int. J. Pharm. (Amsterdam, Neth.) 257, 169–180 (2003).
Heller, I. et al. Identifying the mechanism of biosensing with carbon nanotube transistors. Nano Lett. 8, 591–595 (2008).
Kim, B. & Kim, T.H. Determination of human serum albumin using a single-walled carbon nanotube-FET modified with bromocresol green. Microchim. Acta 183, 1513–1518 (2016).
Jin, J.-H. et al. Real-time selective monitoring of allergenic Aspergillus molds using pentameric antibody-immobilized single-walled carbon nanotube-field effect transistors. RSC Adv. 5, 15728–15735 (2015).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kim, T.H. A simple and real-time sensing of human serum albumin using antibody-modified CNT-FET. BioChip J 11, 116–120 (2017). https://doi.org/10.1007/s13206-016-1204-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13206-016-1204-6