Integrative analysis of miRNA and mRNA profiles in response to myricetin in human endothelial cells
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MicroRNAs (miRNAs) are associated with a diverse range of biological processes, human diseases, and metabolic disorders. Myricetin, which is the most abundant polyphenol class in the human diet, has antioxidative, antiapoptotic, and anti-inflammatory properties. The present study aimed to evaluate whether myricetin modulates miRNA expression. Using microarray analysis, we investigated miRNA expression in human endothelial cells treated with 25 µM and 100 µM of myricetin for 24 hours, and found that 101 and 191 miRNAs, respectively, were differential expressed by at least 1.5-fold. Based on several bioinformatic systems, we also identified signatures of the potential biological processes and signaling pathways that are influenced by dysregulated miRNAs. Therefore, integrating specific patterns of miRNA and mRNA levels may suggest a new mechanism of action of myricetin.
KeywordsEndothelial cells Myricetin Polyphenol miRNA Vascular diseases
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- 4.Lee, S.E. & Park, Y.S. Role of lipid peroxidation-derived alpha, beta-unsaturated aldehydes in vascular dysfunction. Oxid. Med. Cell Longev. 2013, 629028 (2013).Google Scholar
- 22.Katoh, M. Therapeutics targeting angiogenesis: genetics and epigenetics, extracellular miRNAs and signaling networks (Review). Int. J. Mol. Med. 32, 763–767 (2013).Google Scholar