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Aqueous extracts of Anemarrhena asphodeloides stimulate glucagon-like pepetide-1 secretion in enteroendocrine NCI-H716 cells

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Abstract

Anemarrhena asphodeloides (AA), a bitter taste herbal medicine, has been prescribed in traditional oriental medicine to treat diabetes mellitus. Here, AA was extracted and fractionated to investigate its effects on the stimulation of glucagon-like peptide-1 (GLP-1) secretion in enteroendocrine cells. GLP-1 is secreted from the human enteroendocrine L cells to the blood in response to ingested nutrients. Because GLP-1 increases glucose dependent insulin release, it is known as a therapeutic method for the treatment of type II diabetes mellitus. The human enteroendocrine L cell line NCI-H716 expresses various chemoreceptors including the G protein coupled receptor (GPCR). Previous studies suggested that, through the GPCR signaling pathway, the secretion of GLP-1 can be induced in NCI-H716. Accordingly, we studied the GLP-1 stimulation effect of the AA extract and its mode-of-action using the GLP-1 ELISA and microarray. Functional categorization of the microarray data confirmed up or down-regulated gene expressions associated with the GPCR signaling pathway. This study demonstrates that AA extracts have a scientific possibility as a GLP-1 stimulant and thus may have the potential to be a therapeutic herbal medicine for type II diabetes mellitus.

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Authors and Affiliations

  1. College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, Heogi-dong, Dongdaemun-gu, Seoul, 130-701, Korea

    Kang-Hoon Kim, Ki-Suk Kim, Min Hee Shin, Eun Gyeong Jang, Eun Young Kim, Jang-Hoon Lee, Kwang Seok Ahn, Jae-Young Um & Hyeung-Jin Jang

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  1. Kang-Hoon Kim
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  2. Ki-Suk Kim
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  3. Min Hee Shin
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  4. Eun Gyeong Jang
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  5. Eun Young Kim
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  6. Jang-Hoon Lee
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  7. Kwang Seok Ahn
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  8. Jae-Young Um
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  9. Hyeung-Jin Jang
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Correspondence to Hyeung-Jin Jang.

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These authors contributed equally to this work.

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Kim, KH., Kim, KS., Shin, M.H. et al. Aqueous extracts of Anemarrhena asphodeloides stimulate glucagon-like pepetide-1 secretion in enteroendocrine NCI-H716 cells. BioChip J 7, 188–193 (2013). https://doi.org/10.1007/s13206-013-7213-9

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  • DOI: https://doi.org/10.1007/s13206-013-7213-9

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