Observation of a mouse sperm motility in a natural uterine tube-inspired microfluidic channel

Abstract

Bio-inspired engineering has been promisingly spotlighted recently due to the replication of unique natural environments and processes. Fertilization occurs when sperm meets an egg, typically within the uterine tube. Using bio-inspired engineering, we developed natural uterine mimicry for studying sperm motility via the synthetic microfluidic tube system. Mature sperm were collected from the testicular cauda epididymis, and subsequently remained stable for up to 2 days. The primary sperm cells were infused into a single 1.5 cm wrinkle wave patterned microfluidic channel, moving in a prototypical forward sine wave pattern. This study may be useful in understanding the natural behavior of sperm and also may be applied to fertility treatments in the future.

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References

  1. 1.

    Ball, P. Nature’s color tricks. Scientific American 76–79 (2012).

    Google Scholar 

  2. 2.

    Sullivan, T. & Regan, F. Biologically inspired design: Biomimetic design of novel antifouling materials for application to environmental sensing technologies. J. Ocean Tech. 6, 41–54 (2011).

    Google Scholar 

  3. 3.

    Yoo, J.-W. et al. Bio-inspired, bioengineered and biomimetic drug delivery carriers. Nat. Rev. Drug Discov. 10, 521–535 (2011).

    Article  CAS  Google Scholar 

  4. 4.

    Bae, S.J. et al. A gene-networked gel matrix-supported lipid bilayer as a synthetic nucleus system. Langmuir 28, 17036–17042 (2012).

    Article  CAS  Google Scholar 

  5. 5.

    Menguc, Y. Bioinspired materials: Gecko-inspired controllable adhesive structures applied to micromanipulation. Adv. Mater. 6, 1245 (2012).

    Google Scholar 

  6. 6.

    Gadelha, H. et al. Nonlinear instability in flagellar dynamics: a novel modulation mechanism in sperm migration? J. R. Soc. Interface 7, 1689–1697 (2010).

    Article  CAS  Google Scholar 

  7. 7.

    Smith, D.J. et al. Bend propagation in the flagella of migrating human sperm, and its modulation by viscosity. Cell Motil. Cytoskel. 66, 220–236 (2009).

    Article  CAS  Google Scholar 

  8. 8.

    Xie, L. et al. Integration of sperm motility and chemotaxis screening with a microchannel-based device. Clin. Chem. 56, 1270–1278 (2010).

    Article  CAS  Google Scholar 

  9. 9.

    Chen, Y.-A. et al. Analysis of sperm concentration and motility in a microfluidic device. Microfluid Nanofluid 3, 561–570 (2007).

    Article  Google Scholar 

  10. 10.

    Lopez-Garcia, M.D. et al. Sperm motion in a microfluidic fertilization device. Biomed. Microdevices 10, 709–718 (2008).

    Article  CAS  Google Scholar 

  11. 11.

    Cho, C. et al. Haploinsufficiency of protamine-1 or -2 causes infertility in mice. Nat. Genet. 28, 82–86 (2001).

    CAS  Google Scholar 

  12. 12.

    Farrell, P.B. et al. Motility and other characteristics of human sperm can be measured by computer-assisted sperm analysis of samples stained with Hoechst 33342. Fertil Steril 66, 446–453 (1996).

    CAS  Google Scholar 

  13. 13.

    Sleigh, M. Cilia and Flagella. Academic, London (1974).

    Google Scholar 

  14. 14.

    Jana, S. et al. Paramecium swimming in capillary tube. Phys. Fluids. 24, 041901 (2012).

    Article  Google Scholar 

  15. 15.

    Dryl, S. et al. Progress in the study of excitation and response in ciliates. Protoplasma 62, 255–284 (1966).

    Article  CAS  Google Scholar 

  16. 16.

    Fukui, K. et al. Spiral motion of Paramecium caudatum in a small capillary glass tube. J. Eukaryot. Microbiol. 23, 559–563 (1976).

    Article  Google Scholar 

  17. 17.

    Cho, C. et al. Analysis of mouse fertilin in wild-type and fertilin β-/- sperm: evidence of C-terminal modification, / dimerization, and lack of essential role of fertilin α in sperm-egg fusion. Dev. Biol. 222, 289–295 (2000).

    Article  CAS  Google Scholar 

  18. 18.

    Jessamine, M.K. et al. Components for integrated poly (dimethylsiloxane) microfluidic systems. Electrophoresis 23, 3461–3473 (2002).

    Article  Google Scholar 

  19. 19.

    Jana, S. et al. Cilia induced bending of paramecium in microchannels. The IMA Volumes in Mathematics and its Applications 155, 207–215 (2012).

    Article  Google Scholar 

  20. 20.

    Eddins, A. et al. Locomotion of paramecium in patterned environments. SESAPS. ID 109 (2011).

    Google Scholar 

  21. 21.

    Jana, S. et al. Textured boundaries and their effects on ciliary locomotion. Bulletin of the American Physical Society 56, Y9.00008 (2011).

    Google Scholar 

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Correspondence to Soong Ho Um.

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Um, S.H. Observation of a mouse sperm motility in a natural uterine tube-inspired microfluidic channel. BioChip J 7, 46–50 (2013). https://doi.org/10.1007/s13206-013-7107-x

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Keywords

  • Sperm
  • Microfluidics
  • Bio-inspired engineering