Harnessing gravitational, hydrodynamic and negative dielectrophoretic forces for higher throughput cell sorting

Abstract

We present a negative dielectrophoretic (n-DEP) force based high throughput cell sorting system integrating a cantilever-type electrode array in a vertical macro channel to leverage gravity driven flow and eliminate the need for external pumps. The system comprises a macro-sized channel to increase throughput, a cantilever electrode array (L×W×H=150 μm ×500 μm×10 μm) to achieve n-DEP force and high throughput, and a flow regulator to precisely control hydrodynamic force. The hydrodynamic force and the n-DEP force acting on the target cell are evaluated theoretically. In addition, optimal separation conditions are investigated using computational models. Separation conditions are experimentally investigated based on simulation results. Finally, to demonstrate the separation performance of the sorting system, we performed the separation of the human breast cancer cells (MCF 7) from diluted red blood cells (RBCs) under conditions of low voltage (7Vp-p with 500 kHz) and flow rate of 5 μL·min−1. The system can separate MCF 7 cell with 71% separation efficiency in case of the ratio of 1: 60000 (MCF 7: RBCs).

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Correspondence to Byungkyu Kim.

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Lee, J., Kim, Y., Beebe, D.J. et al. Harnessing gravitational, hydrodynamic and negative dielectrophoretic forces for higher throughput cell sorting. BioChip J 6, 229–239 (2012). https://doi.org/10.1007/s13206-012-6305-2

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Keywords

  • Negative dielectrophoresis (n-DEP)
  • Gravity driven flow
  • Macro-sized channel
  • Separation
  • Cantilever-type electrode (CTE) array
  • High throughput sorting (HTS)