Analysis of SSEA1+ vs. SSEA1 fractions of bulk-cultured XENP cell lines


Previously isolated rat extraembryonic endoderm precursor (XENP) cell lines had been characterized after clonal density plating. The arising colonies had consisted of peripheral XENP cells expressing the surface antigen SSEA1 and the transcription factor Oct4, and inner XENP-derived extraembryonic endoderm cells that were nearly negative for SSEA1 and Oct4. We now sorted bulk-cultured XENP cell lines from two rat strains by FACS into SSEA1+ and SSEA1 populations and compared their expression profiles by microarray and RT-PCR. In the bulk cultures, the SSEA1+ fraction was only slightly enriched for Oct4, and also slightly enriched for the visceral endoderm markers, Dab2 and Ihh. Both fractions expressed vascular-associated mesodermal markers (VE-cadherin, Flk1). Thus, in regular-density XENP cell cultures, SSEA1 is not suitable as a stem cell marker, and the XENP cells appear to undergo partial somatic differentiation.

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  1. 1.

    Debeb, B.G. et al. Isolation of Oct4-expressing extra-embryonic endoderm precursor cell lines. PLoS One 4, e7216 (2009).

    Article  Google Scholar 

  2. 2.

    Chazaud, C., Yamanaka, Y., Pawson, T. & Rossant, J. Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathway. Dev. Cell 10, 615–624 (2006).

    Article  CAS  Google Scholar 

  3. 3.

    Muramatsu, T. & Muramatsu, H. Carbohydrate antigens expressed on stem cells and early embryonic cells. Glycoconj. J. 21, 41–45 (2004).

    Article  CAS  Google Scholar 

  4. 4.

    Pennington, J.E., Rastan, S., Roelcke, D. & Feizi, T. Saccharide structures of the mouse embryo during the first eight days of development. J. Embryol. Exp. Morphol. 90, 335–361 (1985).

    CAS  Google Scholar 

  5. 5.

    Harris, E.S. & Nelson, W.J. VE-cadherin: at the front, center, and sides of endothelial cell organization and function. Curr. Opin. Cell Biol. 22, 651–658 (2010).

    Article  CAS  Google Scholar 

  6. 6.

    Yamanaka, Y., Lanner, F. & Rossant, J. FGF signal-dependent segregation of primitive endoderm and epiblast in the mouse blastocyst. Development 137, 715–724 (2010).

    Article  CAS  Google Scholar 

  7. 7.

    Grabarek, J.B. et al. Differential plasticity of epiblast and primitive endoderm precursors within the ICM of the early mouse embryo. Development 139, 129–139 (2012).

    Article  CAS  Google Scholar 

  8. 8.

    Zeineddine, D. et al. Oct-3/4 dose dependently regulates specification of embryonic stem cells toward a cardiac lineage and early heart development. Dev. Cell 11, 535–546 (2006).

    Article  CAS  Google Scholar 

  9. 9.

    Kim, J.S. et al. Array2GO: a simple web-based tool to search gene ontology for analysis of multi genes expression. BioChip J. 4, 320–335 (2010).

    Google Scholar 

  10. 10.

    Zheng, Q. & Wang, X.J. GOEAST: a web-based software toolkit for Gene Ontology enrichment analysis. Nucleic Acids Res. 36 (Web Server issue), W358–W363 (2008).

    Article  CAS  Google Scholar 

  11. 11.

    Huang, D.W., Sherman, B.T. & Lempicki, R.A. Systematic and integrative analysis of large gene lists using DAVID Bioinformatics Resources. Nature Protoc. 4, 44–57 (2009).

    Article  CAS  Google Scholar 

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Correspondence to Bert Binas.

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These authors contributed equally to this work

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Jeong, M., Choi, KW., Kim, S.J. et al. Analysis of SSEA1+ vs. SSEA1 fractions of bulk-cultured XENP cell lines. BioChip J 6, 99–105 (2012).

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  • Stem cells
  • XENP cells
  • Extraembryonic edndoderm
  • SSEA1
  • Oct4
  • Microarray