BioChip Journal

, Volume 6, Issue 1, pp 99–105 | Cite as

Analysis of SSEA1+ vs. SSEA1 fractions of bulk-cultured XENP cell lines

  • Minjin Jeong
  • Kyeng-Won Choi
  • Seung Jun Kim
  • Jungho Kim
  • Bert Binas
Original Research

Abstract

Previously isolated rat extraembryonic endoderm precursor (XENP) cell lines had been characterized after clonal density plating. The arising colonies had consisted of peripheral XENP cells expressing the surface antigen SSEA1 and the transcription factor Oct4, and inner XENP-derived extraembryonic endoderm cells that were nearly negative for SSEA1 and Oct4. We now sorted bulk-cultured XENP cell lines from two rat strains by FACS into SSEA1+ and SSEA1 populations and compared their expression profiles by microarray and RT-PCR. In the bulk cultures, the SSEA1+ fraction was only slightly enriched for Oct4, and also slightly enriched for the visceral endoderm markers, Dab2 and Ihh. Both fractions expressed vascular-associated mesodermal markers (VE-cadherin, Flk1). Thus, in regular-density XENP cell cultures, SSEA1 is not suitable as a stem cell marker, and the XENP cells appear to undergo partial somatic differentiation.

Keywords

Stem cells XENP cells Extraembryonic edndoderm SSEA1 Oct4 Microarray 

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Copyright information

© The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Minjin Jeong
    • 1
  • Kyeng-Won Choi
    • 2
  • Seung Jun Kim
    • 3
  • Jungho Kim
    • 2
  • Bert Binas
    • 1
  1. 1.Division of Molecular and Life ScienceHanyang UniversityAnsanKorea
  2. 2.Department of Life ScienceSogang UniversitySeoulKorea
  3. 3.GenoCheck Inc.AnsanKorea

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