Single cell lysis and DNA extending using electroporation microfluidic device


The purpose of cell lysis is to obtain intracellular substances such as DNA and proteins for analysis. Commonly used methods include chemical (chemical solution decomposition) and physical (electricity or mechanical force). This study proposes an integrated system using an electroporation and microfluidic device made by micro-photolithography to lyse a single cell and stretch its DNA. The PDMS, as the manufacturing material of the microfluidic device, consists of 2 parts: the cell lytic zone, in which the immobilized cells trapped within the dense microstructure are lysed at a single-cell level, and the DNA stretching and recovery zone. This study showed that in a hypotonic environment (75 mM glucose solution), when electric field conditions were 100 Vpp and 1 kHz, the target cell was lysed and its DNA was released into the solution. When injected with proteinase K, the DNA flowed along the rectangular microstructure and was stretched to a length exceeding 840 μm.

This is a preview of subscription content, access via your institution.


  1. 1.

    Kotnik, T., Miklavcic, D. & Slivnik, T. Time course of transmembrance voltage induced by time-varying electric fields-a method for theoretical analysis and its application. Bioelectrochem. 45, 3–16 (1998).

    Article  CAS  Google Scholar 

  2. 2.

    Lu, H., Schmidt, M.A. & Jensen, K.F. A microfluidic electroporation device for cell lysis. Lab Chip 5, 23–29 (2005).

    Article  CAS  Google Scholar 

  3. 3.

    Shin, Y.S. et al. Electrotransfection of mammalian cells using microchannel-type electroporation chip. Anal. Chem. 76, 7045–7052 (2004).

    Article  CAS  Google Scholar 

  4. 4.

    Wang, H.Y. & Lu, C. Electroporation of mammalian cells in a microfluidic channel with geometric variation. Anal. Chem. 78, 5158–5164 (2006).

    Article  CAS  Google Scholar 

  5. 5.

    Lee, D.W. & Cho, Y.H. A continuous electrical cell lysis device using a low dc voltage for a cell transport and rupture. Sens. Actu. B 124, 84–89 (2007).

    Article  Google Scholar 

  6. 6.

    Lin, Y.H. & Lee, G.B. An optically induced cell lysis device using dielectrophoresis. Appl. Phys. Lett. 94, 033901 (2009).

    Article  Google Scholar 

  7. 7.

    Chung, Y.C. et al. Microfluidic chip for high efficiency DNA extraction. Lab Chip 4, 141–147 (2004).

    Article  CAS  Google Scholar 

  8. 8.

    Liu, R.H., Yang, J., Lenigk, R., Bonanno, J. & Grodzinski, P. Self-contained, full integrated biochip for sample preparation, polymerase chain reaction amplification, and DNA microarray detection. Anal. Chem. 76, 1824–1831 (2004).

    Article  CAS  Google Scholar 

  9. 9.

    Lee, C.Y., Lee, G.B., Lin, J.L., Huang, F.C. & Liao, C.S. Integrated microfluidic systems for cell lysis, mixing/pumping and DNA amplification. J. Micromech. Microeng. 15, 1215–1233 (2005).

    Article  CAS  Google Scholar 

  10. 10.

    Teissié, J. & Rols, M.P. An experimental evaluation of the critical potential difference inducing cell membrane electropermeabilization. Biophys. J. 65, 409–413 (1993).

    Article  Google Scholar 

  11. 11.

    Hung, M.-S., Kurosawa, O., Kabata, H. & Washizu, M. Stretching DNA fibers out of a chromosome in solution using electroosmotic flow. J. Chin. Soc. Mech. Eng. 30, 289–295 (2009).

    Google Scholar 

  12. 12.

    Hung, M.-S. & Chen, P.-C. Extending DNA from a single cell using integrated system of electro-osmosis and AFM. J. Med. Biol. Eng. 30, 29–34 (2010).

    Google Scholar 

  13. 13.

    Lee, S.W. & Tai, Y.C. A micro cell lysis device. Sens. Actu. A 73, 74–79 (1999).

    Article  Google Scholar 

  14. 14.

    Neumann, E., Kakorin, S., Tsoneva, I., Nikolova, B. & Tomov, T. Calcium-mediated DNA adsorption to yeast cells and kinetics of cell transformation by electroporation. Biophys. J. 71, 868–877 (1996).

    Article  CAS  Google Scholar 

  15. 15.

    Hu, Z., Kwon, G.H., Kim, C.-B., Kim, D. & Lee, S.- H. Integration of movable structures in PDMS microfluidic channels. BioChip J. 4, 117–122 (2010).

    Article  CAS  Google Scholar 

Download references

Author information



Corresponding author

Correspondence to Min-Sheng Hung.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Hung, MS., Chang, YT. Single cell lysis and DNA extending using electroporation microfluidic device. BioChip J 6, 84–90 (2012).

Download citation


  • Electroporation
  • Cell lysis
  • DNA extending
  • Microfluidic device
  • PDMS