Sensitive and multiplexed analysis of aflatoxins using time-of-flight secondary ion mass spectrometry

Abstract

A rapid and reliable analysis of toxins is prerequisite for food control and human healthcare. Here we demonstrate a simple and multiplexed assay of aflatoxins using time-of-flight secondary ion mass spectrometry (TOF-SIMS). By simply adsorbing either a single analyte or mixed ones onto a gold substrate, the corresponding secondary molecular ions([M+H]+) were clearly observed in a single mass spectrum. As a result of concentration-dependent peak intensity, quantitative and multiplexed analysis of different aflatoxin analogs from corns was accomplished with immunoaffinity column and TOF-SIMS analysis, which showed a good correlation with HPLC data. The detection sensitivity was estimated to be as low as 10 ng mL−1. This approach presented here will find a wide application to detection of low-levels of toxins in a rapid and multiplexed way.

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Correspondence to Young-Pil Kim or Hak-Sung Kim.

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These authors contributed equally to this work.

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Ahn, JH., Jeong, YS., Lee, T.G. et al. Sensitive and multiplexed analysis of aflatoxins using time-of-flight secondary ion mass spectrometry. BioChip J 6, 34–40 (2012). https://doi.org/10.1007/s13206-012-6105-8

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Keywords

  • Aflatoxin
  • TOF-SIMS
  • Quantification
  • Multiplexed analysis