Abstract
Vascular endothelial growth factor (VEGF) protects neuronal cells from amyloid-β(Aβ)-induced neurotoxicity in the brain of Alzheimer’s disease (AD) patient. However, the accumulation of VEGF in amyloid plaques may induce local VEGF deficiency and promote development of the disease. Here we investigated that Aβ1–42 can interact with heparin binding domain (HBD) of VEGF using ProteoChip technology. The ProteoChip-based Aβ-VEGF interaction system has been used for screening of AD inhibitor. An inhibitor of Aβ1–42-VEGF165 interaction using a ProteoChip-based screening method showed suppression of Aβ1–42-induced neurotoxicity in human neuronal cells.
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References
Parihar, M.S. & Hemnani, T. Alzheimer’s disease pathogenesis and therapeutic interventions. J. Clin. Neurosci. 11, 456–467 (2004).
Suh, Y.H. & Checler, F. Amyloid Precursor Protein, Presenilins, and α-Synuclein: Molecular Pathogenesis and Pharmacologycal Applications in Alzheimer’s Disease. Pharmacol. Rev. 54, 469–525 (2002).
Yankner, B.A. Mechanisms of Neuronal Degeneration in Alzheimer’s Disease. Neuron 16, 921–932 (1996).
Lee, S.H. et al. The Change of Expression in Amyloid Precursor Protein mRNA and Amyloid Precursor Protein by Transient Ischemia. J. Korean Neurosurg. Soc. 27, 1481–1489 (1998).
Pike, C.J., Burdick, D., Walencewicz, A.J., Glabe, C.G. & Cotman, C.W. Neurodegeneration Induced by β-Amyloid Peptides in vitro: The Role of Peptide Assembly State. J. Neurosci. 13, 1676–1687 (1993).
Suh, Y.H. An Etiological Role of Amyloidogenic Carboxyl-Terminal Fragments of the β-Amyloid Precursor Protein in Alzheimer’s Disease. J. Neurochem. 68, 1781–1791 (1997).
Yang, S.P., Kwon, B.O., Gho, Y.S. & Chae, C.B. Specific interaction of VEGF165 with β-amyloid, and its protective effect on β-amyloid-induced neurotoxicity. J. Neurochem. 93, 118–127 (2005).
Robinson, C.J. & Stringer, S.E. The splice variants of vascular endothelial growth factor (VEGF) and their receptors. J. Cell Sci. 114, 853–865 (2001).
Yankner, B.A., Duffy, L.K. & Kirschner, D.A. Neurotrophic and neurotoxic effects of amyloid beta protein: reversal by tachykinin neuropeptides. Science 250, 279–282 (1990).
Lee, Y.S. et al. ProteoChip: A highly sensitive protein microarray prepared by a novel method of protein immobilization for application of protein-protein interaction studies. Proteomics 3, 2289–2304 (2003).
Lee, Y.S., Kang, D.K., Chang, S.I., Han, M.H. & Kang, I.C. High-throughput screening of novel peptide inhibitors of an integrin receptor from the hexapeptide library by using a protein microarray chip. J. Biomol. Screen. 9, 687–694 (2004).
Kim, E.Y., Bang, J.Y., Chang, S.I. & Kang, I.C. A novel integrin α5β1 antagonistic peptide, A5-1, screened by Protein Chip system as a potent angiogenesis inhibitor. Biochem. Biophys. Res. Commun. 377, 1288–1293 (2008).
Kim, E.Y. & Kang, I.C. Protein Chip-based Dipeptidyl Peptidase IV Assay System. BioChp J. 3, 249–253 (2009).
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Park, CW., Bang, J.Y., Choi, Y. et al. ProteoChip-based screening system of novel inhibitors against the interaction of VEGF165 and β-Amyloid1–42 . BioChip J 5, 299–303 (2011). https://doi.org/10.1007/s13206-011-5402-y
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DOI: https://doi.org/10.1007/s13206-011-5402-y