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Biomarker analysis of rat livers exposed to different toxic pollutants (VOCs and PAHs) using an antibody array

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Abstract

We have developed an antibody microarray chip for biomarker analysis in rat livers exposed to five different toxic pollutants: volatile organic compounds (VOCs), dichloromethane, ethylbenzene, and trichloroethylene; and polycyclic aromatic hydrocarbons (PAHs), benzoanthracene and phenanthrene. The antibody chip consisted of antibodies against different biomarkers: alpha-2μ globulin, glutathione S transferase (GST)-α, Hemoglobin β, and Phenylalanine hydroxylase. VOC- and PAH-treated rat livers showed differential expression patterns of the biomarker proteins. These results demonstrate that a protein chipbased antibody array is useful for the analysis of biomarkers induced by environmental toxic pollutants and can be a powerful tool to study the mechanisms of pollutants in biological samples.

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Authors and Affiliations

  1. Department of Biological Science and BioChip Research Center, Hoseo University & Innopharmascreen Inc., Asan, 336-795, Korea

    Eung-Yoon Kim & In-Cheol Kang

  2. Department of Medicinal Biotechnology, SoonChunHyang University, Asan, 336-745, Korea

    Mi-Young Lee

  3. Division of Molecular and Life Science, Hanyang University & Geno Check Co. Ltd., Sangnok-gu, Ansan, Gyeonggi-do, Korea

    Seung Yong Hwang

Authors
  1. Eung-Yoon Kim
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  2. Mi-Young Lee
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  3. Seung Yong Hwang
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  4. In-Cheol Kang
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Correspondence to In-Cheol Kang.

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Kim, EY., Lee, MY., Hwang, S.Y. et al. Biomarker analysis of rat livers exposed to different toxic pollutants (VOCs and PAHs) using an antibody array. BioChip J 4, 173–178 (2010). https://doi.org/10.1007/s13206-010-4302-x

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  • DOI: https://doi.org/10.1007/s13206-010-4302-x

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