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Comparison of the performance of the PANArray™ HPV test and DNA chip test for genotyping of human papillomavirus in cervical swabs

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Abstract

The aim of this study was to evaluate the recently developed PANArray™ Human papilloma virus (HPV) kit for detection and genotyping of 19 high-risk and 13 low-risk HPV types, and compare it with the commercially available DNA chip kit genotyping of 24 HPVs. We collected cervical swabs from 741 patients with various stages of invasive cervical carcinoma being treated at the Samsung Medical Center. The overall HPV positivity rate was 73% using PANArrayTM HPV and 72.1% with the DNA chip, and no statistically significant differences were found with respect to the cytology grade. Comparing the results of the two chips, concordant results were found in 637/741 samples (85.9%), compatible in 69/741 (9.3%) and discordant in 35/741 (4.7%). Type-specific sequencing analysis of all samples revealed a 99.7% confirmation of PANArrayTM HPV genotyping results, compared to 91.0% of DNA chip. The PANArrayTM HPV test thus proved to be highly sensitive and accurate even when multiple HPV infections were present.

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Authors and Affiliations

  1. Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, 135-710, Korea

    Hye Jong Song, Sang Yong Song & Dae Shick Kim

  2. Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, 135-710, Korea

    Jeong Won Lee, Byoung Gie Kim & Duk Soo Bae

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  1. Hye Jong Song
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  2. Jeong Won Lee
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  3. Byoung Gie Kim
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  4. Sang Yong Song
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  5. Duk Soo Bae
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  6. Dae Shick Kim
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Correspondence to Dae Shick Kim.

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Song, H.J., Lee, J.W., Kim, B.G. et al. Comparison of the performance of the PANArray™ HPV test and DNA chip test for genotyping of human papillomavirus in cervical swabs. BioChip J 4, 167–172 (2010). https://doi.org/10.1007/s13206-010-4301-y

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  • DOI: https://doi.org/10.1007/s13206-010-4301-y

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