Abstract
The present report communicates the first complete genome sequence of S-type Mycobacterium avium subsp. paratuberculosis, isolated from an organised goat herd in Uttar Pradesh, India. Bacteria were isolated in pure culture on Herrold’s egg yolk medium (HEYM) slants containing mycobactin J from the faecal sample collected per-rectally from a clinical diseased goat, and next-generation sequencing (NGS) revealed that the genome sequence length of the isolated strain named MAP-Gt-9 is 4,509,428 bp with no plasmid DNA, with a GC content of 69.5%, an N50 value of 125,474 bp, and an L50 value of 12, containing 4235 coding DNA sequences (CDSs), 44 tRNAs, 3 ncRNAs and 1 each 5S, 16S, 23S rRNA genes.
Data availability
This whole-genome shotgun project has been deposited at DDBJ/ENA/GenBank under the accession number JAHWGM000000000and the BioProject accession number PRJNA743192. The version described in this paper is version JABBHV000000000. The sequences have been submitted to the Sequence Read Archive (SRA) under the accession number SRR15101044 and are available at following https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA743192.
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We thank the Vice Chancellor, U.P. Veterinary University (DUVASU), Mathura for providing the necessary funds for the study.
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The study was approved with approval no. IAEC/18/25 by Institute Animal Ethics Committee, Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan (DUVASU), Mathura, Uttar Pradesh, India registered with Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), Government of India with registration no. 386/PO/ReBi/SL/01/CPCSEA.
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Singh, V.K., Das, C., Kumar, A. et al. Complete genome sequence of S-type Mycobacterium avium subsp. paratuberculosis isolated from an organized goat herd in India. 3 Biotech 14, 38 (2024). https://doi.org/10.1007/s13205-023-03896-2
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DOI: https://doi.org/10.1007/s13205-023-03896-2