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circ_UTRN inhibits ferroptosis of ARJ21 cells to attenuate acute pancreatitis progression by regulating the miR-760-3p/FOXO1/GPX4 axis

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Abstract

Aim

To explore the function of circ_UTRN in acute pancreatitis (AP).

Methods

After exposing AR42J cells to caerulein, the levels of circ_UTRN, miR-760-3p, and glutathione peroxidase 4 (GPX4) were determined by quantitative polymerase chain reaction. Additionally, GPX4 and forkhead box O1 (FOXO1) protein levels were assessed by western blot. The levels of oxidative stress and ferroptosis in the supernatant of the treated AR42J cells were also assessed using commercial kits.

Results

circ_UTRN inhibited caerulein-induced oxidative stress and ferroptosis by binding with miR-760-3p. Additionally, miR-760-3p directly targeted FOXO1, thereby regulating GPX4 levels. Furthermore, GPX4 knockdown abolished the effect of miR-760-3p downregulation in AP.

Conclusion

circ_UTRN inhibited oxidative stress and ferroptosis by regulating the miR-760-3p/FOXO1/GPX4 axis. This is a potential new treatment strategy for AP.

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Availability of data and material

All data generated or analyzed during this study are included in this published article

Abbreviations

AP:

Acute pancreatitis

UTRN:

Utrophin

CircRNAs:

Circular RNAs

CeRNA:

Competing endogenous RNA

GPX4:

Glutathione peroxidase 4

FOXO1:

Forkhead box O1

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Acknowledgements

The funding body did not have any role in the design of the study, the collection, analysis and interpretation of the data or the writing of the manuscript.

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Author notes

  1. Lanlan Wei and Bowen Li are co-first authors.

Authors and Affiliations

  1. Department of Respiratory and Critical Care Medicine, Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University, Zhuzhou, 412000, Hunan Province, People’s Republic of China

    Lanlan Wei

  2. Jishou University School of Medicine, Jishou, 416000, Hunan Province, People’s Republic of China

    Bowen Li

  3. Department of Ultrasonography, Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University, Zhuzhou, 412000, Hunan Province, People’s Republic of China

    Jing Long

  4. Emergency Department, Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University, No. 116, Changjiang South Road, Tianyuan District, Zhuzhou, 412000, Hunan Province, People’s Republic of China

    Yanping Fu

  5. Department of Hepatobiliary Surgery, Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University, Tianyuan District, No. 116, Changjiang South Road, Zhuzhou, 412007, Hunan Province, People’s Republic of China

    Bin Feng

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  1. Lanlan Wei
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Correspondence to Yanping Fu or Bin Feng.

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Wei, L., Li, B., Long, J. et al. circ_UTRN inhibits ferroptosis of ARJ21 cells to attenuate acute pancreatitis progression by regulating the miR-760-3p/FOXO1/GPX4 axis. 3 Biotech 14, 84 (2024). https://doi.org/10.1007/s13205-023-03886-4

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