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Isolation of a marine-derived yeast with potential applications in industrial nitrite utilizing

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Abstract

The nitrite efficient utilization microorganism Wickerhamomyces anomalus RZWP01 was identified. Using nitrite and ammonium as the sole nitrogen source, the nitrogen removal rate of W. anomalus RZWP01 was 97.4% and 87.1%, respectively. W. anomalus RZWP01 grew well in the nitrite medium with glucose or xylose as the only carbon source. However, the W. anomalus RZWP01 cannot live on the nitrite medium with lactose, citric acid, and methanol as the only carbon source. The maximal cell concentration occurred in the nitrite medium with glucose as the only carbon source at a C/N ratio of 20 for 48 h, reaching 8.92 × 108 cell mL−1. W. anomalus RZWP01 was the first reported yeast that can efficiently utilize nitrite. The isolation and identification of W. anomalus RZWP01 enriched the microbial resources of nitrite-degrading microorganisms and provided functional microorganisms for the water treatment of sustainable aquaculture.

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The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.

References

  • Broman E, Zilius M, Samuiloviene A, Vybernaite-Lubiene I, Politi T, Klawonn I, Voss M, Nascimento FJA, Bonaglia S (2021) Active DNRA and denitrification in oxic hypereutrophic waters. Water Res 194:116954

    Article  CAS  PubMed  Google Scholar 

  • Butala NS, Falkinham JO (2018) Nitrate and nitrite reductase activities of Mycobacterium avium. Int J Mycobacteriol 7(4):328–331

    Article  CAS  PubMed  Google Scholar 

  • Cao S, Zhou Y (2019) New direction in biological nitrogen removal from industrial nitrate wastewater via anammox. Appl Microbiol Biotechnol 103(18):7459–7466

    Article  CAS  PubMed  Google Scholar 

  • Chen Z, Wang X, Chen X, Chen J, Feng X, Peng X (2018) Nitrogen removal via nitritation pathway for low-strength ammonium wastewater by adsorption, biological desorption and denitrification. Bioresour Technol 267:541–549

    Article  CAS  PubMed  Google Scholar 

  • Chen S, He S, Wu C, Du D (2019) Characteristics of heterotrophic nitrification and aerobic denitrification bacterium Acinetobacter sp. T1 and its application for pig farm wastewater treatment. J Biosci Bioeng 127(2):201–205

    Article  CAS  PubMed  Google Scholar 

  • Chen L, Lin J, Pan D, Ren Y, Zhang J, Zhou B, Chen L, Lin J (2020) Ammonium removal by a newly isolated heterotrophic nitrification-aerobic denitrification bacteria Pseudomonas stutzeri SDU10 and its potential in treatment of piggery wastewater. Curr Microbiol 77(10):2792–2801

    Article  CAS  PubMed  Google Scholar 

  • Cherchi C, Onnis-Hayden A, El-Shawabkeh I, Gu AZ (2009) Implication of using different carbon sources for denitrification in wastewater treatments. Water Environ Res 81(8):788–799

    Article  CAS  PubMed  Google Scholar 

  • Conthe M, Lycus P, Arntzen M, Ramos da Silva A, Frostegård Å, Bakken LR, Kleerebezem R, van Loosdrecht MCM (2019) Denitrification as an N(2)O sink. Water Res 151:381–387

    Article  CAS  PubMed  Google Scholar 

  • Díaz-García C, Martínez-Sánchez JJ, Maxwell BM, Franco JA, Álvarez-Rogel J (2021) Woodchip bioreactors provide sustained denitrification of brine from groundwater desalination plants. J Environ Manage 289:112521

    Article  PubMed  Google Scholar 

  • Fang J, Liao S, Zhang S, Li L, Tan S, Li W, Wang A, Ye J (2021) Characteristics of a novel heterotrophic nitrification-aerobic denitrification yeast, Barnettozyma californica K1. Bioresour Technol 339:125665

    Article  CAS  PubMed  Google Scholar 

  • Gupta RK, Poddar BJ, Nakhate SP, Chavan AR, Singh AK, Purohit HJ, Khardenavis AA (2022) Role of heterotrophic nitrifiers and aerobic denitrifiers in simultaneous nitrification and denitrification process: a nonconventional nitrogen removal pathway in wastewater treatment. Lett Appl Microbiol 74(2):159–184

    Article  CAS  PubMed  Google Scholar 

  • Hang Q, Wang H, Chu Z, Ye B, Li C, Hou Z (2016) Application of plant carbon source for denitrification by constructed wetland and bioreactor: review of recent development. Environ Sci Pollut Res Int 23(9):8260–8274

    Article  CAS  PubMed  Google Scholar 

  • Haridas D, Biffinger JC, Boyd TJ, Fulmer PA, Hamdan LJ, Fitzgerald LA (2017) Laboratory growth of denitrifying water column microbial consortia from deep-sea shipwrecks in the northern Gulf of Mexico. F1000Res 6:1834

    Article  PubMed  Google Scholar 

  • Lee YY, Choi H, Cho KS (2019) Effects of carbon source, C/N ratio, nitrate, temperature, and pH on N(2)O emission and functional denitrifying genes during heterotrophic denitrification. J Environ Sci Health A Tox Hazard Subst Environ Eng 54(1):16–29

    Article  CAS  PubMed  Google Scholar 

  • Li B, Lv R, Xiao Y, Hu W, Mai Y, Zhang J, Lin L, Hu X (2019) A novel nitrite-base aerobic denitrifying bacterium Acinetobacter sp, YT03 and its transcriptome analysis. Front Microbiol 10:2580

    Article  PubMed  PubMed Central  Google Scholar 

  • Liu Y, Hu T, Song Y, Chen H, Lv Y (2015) Heterotrophic nitrogen removal by Acinetobacter sp. Y1 isolated from coke plant wastewater. J Biosci Bioeng 120(5):549–554

    Article  CAS  PubMed  Google Scholar 

  • Liu T, Lu Y, Zheng M, Hu S, Yuan Z, Guo J (2021) Efficient nitrogen removal from mainstream wastewater through coupling Partial Nitritation, Anammox and Methane-dependent nitrite/nitrate reduction (PNAM). Water Res 206:117723

    Article  CAS  PubMed  Google Scholar 

  • Martínez-Espinosa C, Sauvage S, Al Bitar A, Green PA, Vörösmarty CJ, Sánchez-Pérez JM (2021) Denitrification in wetlands: a review towards a quantification at global scale. Sci Total Environ 754:142398

    Article  PubMed  Google Scholar 

  • Michalski R, Pecyna-Utylska P, Kernert J (2021) Determination of ammonium and biogenic amines by ion chromatography. A review. J Chromatogr A 1651:462319

    Article  CAS  PubMed  Google Scholar 

  • Nelson MB, Martiny AC, Martiny JB (2016) Global biogeography of microbial nitrogen-cycling traits in soil. Proc Natl Acad Sci U S A 113(29):8033–8040

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Putra I, Effendi I, Lukistyowati I, Tang UM, Fauzi M, Suharman I, Muchlisin ZA (2020) Effect of different biofloc starters on ammonia, nitrate, and nitrite concentrations in the cultured tilapia Oreochromis niloticus system. F1000Res 9:293

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Saldana DJ, Jones CM, Gehman AM, Heinrichs AJ (2019) Effects of once- versus twice-a-day feeding of pasteurized milk supplemented with yeast-derived feed additives on growth and health in female dairy calves. J Dairy Sci 102(4):3654–3660

    Article  CAS  PubMed  Google Scholar 

  • Shukla S, Rajta A, Setia H, Bhatia R (2020) Simultaneous nitrification-denitrification by phosphate accumulating microorganisms. World J Microbiol Biotechnol 36(10):151

    Article  CAS  PubMed  Google Scholar 

  • Sun Y, De Vos P, Willems A (2018) Influence of nitrate and nitrite concentration on N(2) O production via dissimilatory nitrate/nitrite reduction to ammonium in Bacillus paralicheniformis LMG 6934. Microbiologyopen 7(4):e00592

    Article  PubMed  PubMed Central  Google Scholar 

  • Wang J, Chu L (2016) Biological nitrate removal from water and wastewater by solid-phase denitrification process. Biotechnol Adv 34(6):1103–1112

    Article  CAS  PubMed  Google Scholar 

  • Wang QH, Yu LJ, Liu Y, Lin L, Lu RG, Zhu JP, He L, Lu ZL (2017) Methods for the detection and determination of nitrite and nitrate: a review. Talanta 165:709–720

    Article  CAS  PubMed  Google Scholar 

  • Xu Z, Dai X, Chai X (2018) Effect of different carbon sources on denitrification performance, microbial community structure and denitrification genes. Sci Total Environ 634:195–204

    Article  CAS  PubMed  Google Scholar 

  • Yang M, Lu D, Qin B, Liu Q, Zhao Y, Liu H, Ma J (2018) Highly efficient nitrogen removal of a coldness-resistant and low nutrient needed bacterium, Janthinobacterium sp. M-11. Bioresour Technol 256:366–373

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This study was supported by the China Postdoctoral Science Foundation (2021M691852), the Science Foundation of Rizhao (RZ2021ZR16), Rizhao Polytechnic Cultivation Plan Project (2022K01).

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Authors and Affiliations

  1. Department of Marine Technology, Rizhao Polytechnic, Rizhao, 276826, Shandong, China

    Zhen Ding, Zhongping Xu, Faxin Zheng, Ning Fu & Meiling An

  2. Joint Research and Development Centre of Biotechnology, RETAD, Rizhao, 276826, China

    Zhen Ding, Li Zhang & Hongli Xu

  3. State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, China

    Zhen Ding & Lushan Wang

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  1. Zhen Ding
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  2. Li Zhang
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  4. Hongli Xu
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  6. Ning Fu
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  7. Lushan Wang
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  8. Meiling An
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Contributions

All the authors participated in the content of the article. ZD, LZ, and MA conceived and designed the research. ZD, ZX, and HX performed the experiments and analyzed the data. FZ, NF, and LW contributed to new reagents or analytical tools. ZD and MA wrote the paper manuscript. All authors read and approved the manuscript.

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Correspondence to Meiling An.

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Ding, Z., Zhang, L., Xu, Z. et al. Isolation of a marine-derived yeast with potential applications in industrial nitrite utilizing. 3 Biotech 14, 29 (2024). https://doi.org/10.1007/s13205-023-03866-8

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