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Transcriptomics reveals key genes responsible for functional diversity in pectoralis major muscles of native black Kadaknath and broiler chicken

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Abstract

RNA sequencing-based expression profiles from pectoralis major muscles of black meat (Kadaknath) and white meat (broiler) chicken were compared to identify differentially expressed genes. A total of 156 genes with log2 fold change ≥  ± 2.0 showed higher expression in Kadaknath and 68 genes were expressed at a lower level in comparison to broiler. Significantly enriched biological functions of up-regulated genes in Kadaknath were skeletal muscle cell differentiation, regulation of response to reactive oxygen, positive regulation of fat cell differentiation and melanosome. Significant ontology terms up-regulated in broiler included DNA replication origin binding, G-protein coupled receptor signaling pathway and chemokine activity. Highly inter-connected differentially expressed genes in Kadaknath (ATFs, C/EPDs) were observed to be important regulators of cellular adaptive functions, while in broiler, the hub genes were involved in cell cycle progression and DNA replication. The study is an attempt to get an insight into the transcript diversity of pectoralis major muscles of Kadaknath and broiler chicken.

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Data availability

All data supporting this study was generated by ICAR -NBAGR. The RNAseq data generated in the study has been deposited in the NCBI (Short Read Archive Bioproject PRJNA832030).Declarations.

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Acknowledgements

This funds for this study were provided by ICAR- CABin Scheme, New Delhi. We express our gratitude to Director, ICAR- National Bureau of Animal Genetic Resources (NBAGR), Karnal and Indian Council of Agricultural Research (ICAR), New Delhi for providing essential facilities.

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Authors and Affiliations

  1. ICAR-National Bureau of Animal Genetic Resources, Karnal, India

    Reena Arora, Rekha Sharma, Sonika Ahlawat, Pooja Chhabra, Ashish Kumar, Mandeep Kaur & Ramesh Kumar Vijh

  2. ICAR-Indian Agricultural Statistics Research Institute, New Delhi, India

    Shashi Bhushan Lal, Dwijesh Chandra Mishra, Md. Samir Farooqi & Sudhir Srivastava

  3. Animal Biotechnology Division, G T Road By-Pass, P O Box 129, Karnal, Haryana, 132001, India

    Reena Arora

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  1. Reena Arora
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Contributions

RA, SA: conceptualization and supervision. RA, SA, AK, MK: resources and data curation. RA, PC, RKV, SBL, DCM, MSF and SS: formal analyses; RA, RS: writing—review and editing. All authors reviewed the manuscript.

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Correspondence to Reena Arora.

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Arora, R., Sharma, R., Ahlawat, S. et al. Transcriptomics reveals key genes responsible for functional diversity in pectoralis major muscles of native black Kadaknath and broiler chicken. 3 Biotech 13, 253 (2023). https://doi.org/10.1007/s13205-023-03682-0

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