Abstract
Acid-producing bacteria are one kind of crucial species for Baijiu fermentation. The strain BJN0003 with the ability of producing butyric acid was isolated from the cellar mud of Baijiu, and the 16S rRNA gene sequence similarity was 94.2% to its most closely related type species Caproicibacterium lactiferaments JNU-WLY1368T, less than the threshold value of 94.5% for distinguishing genera. Furthermore, the genome of BJN0003 showed a length of 2,458,513 bp and a DNA G + C content of 43.3% through high throughput sequence. BJN0003 exhibited whole-genome average nucleotide identity value of 68.9% to the most closely related species, while the whole-genome digital DNA-DNA hybridization value was only 23.1%, which were both below the delineation thresholds of species. These results indicated BJN0003 could represent a potential novel species of a new genus of the family Oscillospiraceae, and was proposed the name as Butyriproducens baijiuensis. In addition, gene annotation and metabolic analysis showed that BJN0003 harbored the metabolic pathway of converting glucose to butyric acid. The discovery of the new species provided bacterial resource for Baijiu production and the revealing of genetic characteristics would promote the investigation of acid synthesis during Baijiu manufacturing process.
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Data availability
The genome sequence was submitted to National Center for Biotechnology Information with GenBank accession No. CP111108.1. The BioProject accession number was PRJNA899669, and BioSample accession number was SAMN31666078.
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Funding
This work was supported by National Natural Science Foundation of China (No. 32072165, 31830069), Beijing Municipal Natural Science Foundation & Beijing Municipal Education Commission (No. KM202110011003, KZ202010011018).
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MW: methodology, investigation, and data curation. YX: methodology, investigation, data curation, writing—original draft, funding acquisition, and supervision. MD: data curation and methodology. WL: methodology and investigation. CZ: data curation and methodology. XL: review and editing, funding acquisition, and supervision. BS: review and editing, and supervision.
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Wu, M., Xu, Y., Dai, M. et al. Butyriproducens baijiuensis BJN0003: a potential new member of the family Oscillospiraceae isolated from Chinese Baijiu. 3 Biotech 13, 205 (2023). https://doi.org/10.1007/s13205-023-03624-w
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DOI: https://doi.org/10.1007/s13205-023-03624-w