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Extraction of biodiesel from vegetable waste hydrolysates and evaluation of its engine performance and emission characteristics

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Abstract

Recently, microbial oil has become one of the promising next-generation feedstocks for producing biodiesel. While microbial oil can be extracted from different sources, there is only limited work on microbial production from fruits and vegetables. In this work, biodiesel was extracted through a two-step process: microbial conversion of vegetable waste into microbial oil using Lipomyces starkeyi, followed by transesterification of microbial oil into biodiesel. The lipid accumulation, composition of microbial oil, and the fuel properties of biodiesel were evaluated. The microbial oil consisted mainly of C16:0, C18:0 and C18:1, which were close to the properties of palm oil. The fuel properties of biodiesel comply with the EN14214:2012 standard. Thus, the vegetable waste can be a good biodiesel feedstock. Three blends (MOB10, MOB20 and MOB30 with 10, 20, and 30% of biodiesel) were tested for engine performance and emission characteristics in a 3.5 kW VCR research engine. At full load, MOB20 reduced the pollutant emissions of CO and HC by 47.8% and 33.2% with the penalty of increased NOx by 3.9%, while BTE reduced by 0.8% with the increased BSFC by 5.2%. Thus, the addition of vegetable waste biodiesel blends reduced the emissions of CO and HC significantly with slight reduction of brake thermal efficiency.

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References

  • Adi DD, Oduro I, Simpson BK (2019) Biological and microbial technologies for the transformation of fruits and vegetable wastes. In: Simpson BK, Aryee ANA, Toldrá F (eds) Ch 17 of byproducts from agriculture and fisheries. Wiley, pp 403–420. https://doi.org/10.1002/9781119383956.ch17

  • Al-Widyan MI, Al-Shyoukh AO (2002) Experimental evaluation of the transesterification of waste palm oil into biodiesel. Biorespur Technol 85(3):253–256

    Article  CAS  Google Scholar 

  • Angerbauer C, Siebenhofer M, Mittelbach M, Guebitz GM (2008) Conversion of sewage sludge into lipids by Lipomyces starkeyi for biodiesel production. Bioresour Technol 99(8):3051–3056

    Article  CAS  PubMed  Google Scholar 

  • Azad AK, Yousuf A, Ferdoush A, Hasan MM, Karim MR, Jahan A (2014) Production of microbial lipids from rice straw hydrolysates by Lipomyces starkeyi for biodiesel synthesis. J Microb Biochem Technol S 8(2):1–6

    Google Scholar 

  • Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37(8):911–917

    Article  CAS  PubMed  Google Scholar 

  • Chatterjee B, Mazumder D (2020) New approach of characterizing fruit and vegetable waste (FVW) to ascertain its biological stabilization via two-stage anaerobic digestion (AD). Biomass Bioenerg 139:105594

    Article  CAS  Google Scholar 

  • Chatterjee S, Mohan SV (2018) Microbial lipid production by Cryptococcus curvatus from vegetable waste hydrolysate. Biores Technol 254:284–289

    Article  CAS  Google Scholar 

  • Di-Fidio N, Dragoni F, Antonetti C, De Bari I, Galletti AMR, Ragaglini G (2020) From paper mill waste to single cell oil: enzymatic hydrolysis to sugars and their fermentation into microbial oil by the yeast Lipomyces starkeyi. Biores Technol 315:123790

    Article  CAS  Google Scholar 

  • Diwan B, Parkhey P, Gupta P (2018) From agro-industrial wastes to single cell oils: a step towards prospective biorefinery. Folia Microbiol 63(5):547–568

    Article  CAS  Google Scholar 

  • FAO (2011) Global food losses and food waste-extent, causes and prevention. In: International Congress SAVE FOOD!, Rome, pp 1–37

  • FAO (2015) Global initiatives on food loss and waste reduction. http://www.fao.org/save-food. Accessed 26 Aug 2017, p 8

  • Fidio ND, Dragoni F, Antonetti C, De Bari I, Galletti AMR, Ragaglini G (2020) From paper mill waste to single cell oil: enzymatic hydrolysis to sugars and their fermentation into microbial oil by the yeast Lipomyces starkeyi. Bioresour Technol. https://doi.org/10.1016/j.biortech.2020.123790

    Article  PubMed  Google Scholar 

  • Ganjehkaviri A, Mohd Jaafar MN, Hosseini SE, Musthafa AB (2016) Performance evaluation of palm oil-based biodiesel combustion in an oil burner. Energies 9(2):97

    Article  Google Scholar 

  • Godfray HCJ, Beddington JR, Crute IR, Haddad L, Lawrence D, Muir JF, Pretty J, Robinson S, Thomas SM, Toulmin C (2010) Food security: the challenge of feeding 9 billion people. Science 327:812–818

    Article  CAS  PubMed  Google Scholar 

  • Huang C, Chen XF, Yang XY, Xiong L, Lin XQ, Yang J, Chen XD (2014) Bioconversion of corncob acid hydrolysate into microbial oil by the oleaginous yeast Lipomyces starkeyi. Appl Biochem Biotechnol 172(4):2197–2204

    Article  CAS  PubMed  Google Scholar 

  • Islam MA, Yousuf A, Karim A, Pirozzi D, Khan MR, Ab Wahid Z (2018) Bioremediation of palm oil mill effluent and lipid production by Lipomyces starkeyi: a combined approach. J Clean Prod 172:1779–1787

    Article  CAS  Google Scholar 

  • Jeevahan J, Mageshwaran G, Joseph GB, Raj RBD, Thamarai Kannan R (2017) Various strategies for reducing Nox emissions of biodiesel fuel used in conventional diesel engines: a review. Chem Eng Commun 204(10):1202–1223

    Article  CAS  Google Scholar 

  • Khalid A, Osman SA, Jaat M, Mustaffa N, Basharie SM, Manshoor B (2013) Performance and emissions characteristics of diesel engine fuelled by biodiesel derived from palm oil. Appl Mech Mater 315:517–522

    Article  Google Scholar 

  • Liu LP, Zong MH, Hu Y, Li N, Lou WY, Wu H (2017) Efficient microbial oil production on crude glycerol by Lipomyces starkeyi AS 2.1560 and its kinetics. Process Biochem 58:230–238

    Article  CAS  Google Scholar 

  • Martani F, Maestroni L, Torchio M, Ami D, Natalello A, Lotti M, Branduardi P (2020) Conversion of sugar beet residues into lipids by Lipomyces starkeyi for biodiesel production. Microbial Cell Fact 19(1):1–13

    Article  Google Scholar 

  • Mauzerall DL, Sultan B, Kim N, Bradford DF (2005) NOx emissions from large point sources: variability in ozone production, resulting health damages and economic costs. Atmos Environ 39(16):2851–2866

    Article  CAS  Google Scholar 

  • Meng X, Yang J, Xu X, Zhang L, Nie Q, Xian M (2009) Biodiesel production from oleaginous microorganisms. Renew Energy 34(1):1–5

    Article  Google Scholar 

  • Ratledge C (1987) Lipid biotechnology: a wonderland for the microbial physiologist. JAOCS 64(12):1647–1656

    Article  CAS  Google Scholar 

  • Ratledge C (1991) Microorganisms for lipids. Acta Biotechnol 11(5):429–438

    Article  CAS  Google Scholar 

  • Ravi PP, Lindner J, Oechsner H, Lemmer A (2018) Effects of target pH-value on organic acids and methane production in two-stage anaerobic digestion of vegetable waste. Bioresour Technol 247:96–102

    Article  CAS  PubMed  Google Scholar 

  • Sivasaravanan S, Booma Devi P, Nagaraj M, Jeevahan JJ, Joseph GB (2019) Influence of rice husk nanoparticles on engine performance and emission characteristics of diesel and neem oil biodiesel blends in a single cylinder diesel engine. Energy Sources Part A Recov Util Environ Effects. https://doi.org/10.1080/15567036.2019.1693666

    Article  Google Scholar 

  • Sutanto S, Zullaikah S, Tran-Nguyen PL, Ismadji S, Ju Y-H (2018) Lipomyces starkeyi : Its current status as a potential oil producer. Fuel Process Technol 177:39–55

    Article  CAS  Google Scholar 

  • Takaku H, Matsuzawa T, Yaoi K, Yamazaki H (2020) Lipid metabolism of the oleaginous yeast Lipomyces starkeyi. Appl Microbiol Biotechnol. https://doi.org/10.1007/s00253-020-10695-9

    Article  PubMed  Google Scholar 

  • Tsakona S, Kopsahelis N, Chatzifragkou A, Papanikolaou S, Kookos IK, Koutinas AA (2014) Formulation of fermentation media from flour-rich waste streams for microbial lipid production by Lipomyces starkeyi. J Biotechnol 189:36–45

    Article  CAS  PubMed  Google Scholar 

  • Tsouko E, Papanikolaou S, Koutinas AA (2016) Production of fuels from microbial oil using oleaginous microorganisms. In: Handbook of biofuels production, pp 201–236

  • Turcotte G, Kosaric N (1989) The effect of C/N ratio on lipid production by Rhodosporidium toruloides ATCC 10788. Biotech Lett 11(9):637–642

    Article  CAS  Google Scholar 

  • Wild R, Patil S, Popović M, Zappi M, Dufreche S, Bajpai R (2010) Lipids from Lipomyces starkeyi. Food Technol Biotechnol 48(3):329–335

    CAS  Google Scholar 

  • Xavier MCA, Coradini ALV, Deckmann AC, Franco TT (2017) Lipid production from hemicellulose hydrolysate and acetic acid by Lipomyces starkeyi and the ability of yeast to metabolize inhibitors. Biochem Eng J 118:11–19

    Article  CAS  Google Scholar 

  • Yousuf A, Sannino F, Addorisio V, Pirozzi D (2010) Microbial conversion of olive oil mill wastewaters into lipids suitable for biodiesel production. J Agric Food Chem 58(15):8630–8635

    Article  CAS  PubMed  Google Scholar 

  • Zambiazi RC, Przybylski R, Zambiazi MW, Mendonça CB (2007) Fatty acid composition of vegetable oils and fats. Boletim Do Centro De Pesquisa De Processamento De Alimentos 25(1):111–120

    CAS  Google Scholar 

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  1. School of Mechanical Engineering, Sathyabama Institute of Science and Technology, Chennai, Tamil Nadu, India

    Jeya Jeevahan Jayaraj

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  1. Jeya Jeevahan Jayaraj
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Jayaraj, J.J. Extraction of biodiesel from vegetable waste hydrolysates and evaluation of its engine performance and emission characteristics. 3 Biotech 13, 188 (2023). https://doi.org/10.1007/s13205-023-03611-1

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