Abstract
Pisolithus albus is a ubiquitous ectomycorrhizal fungus that establishes symbiosis with a wide range of woody plants around the globe. The symbiotic association of this fungus plays a crucial role in the nutrient cycling of their host plants and enables them to thrive in adverse environmental conditions. Based on its ecological importance and lack of genomic studies, whole-genome sequencing was carried out to analyze P. albus sequences through an Illumina HiSeq X system. The functional annotations were performed against various databases to explore genomic patterns and traits possibly attributing to its specialization. Comparative genomics of P. albus with phylogenetically related Pisolithus microcarpus and Pisolithus tinctorius (only available genomes of Pisolithus at NCBI till now) led to the identification of their unique and shared basic functional and stress adaptation capabilities. The de novo assembled genome of 56.15 Mb with 91.8% BUSCO completeness is predicted to encode 23,035 genes. The study is aimed to generate solid genomic data resources for P. albus, forming the theoretical basis for future transcriptomic, proteomic and metabolomic studies.
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Data Availability
The datasets generated during and/or analyzed during the current study are available in the GenBank repository by the corresponding author.
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Authors are thankful to Thapar Institute of Engineering and Technology for providing the facilities.
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EC performed the whole analysis and wrote the first draft. PS curated and mentored bioinformatics analysis. KMM and MSR supervised the project. PS, KMM and MSR proofread the manuscript before all the authors agreed to the manuscript.
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Chot, E., Suravajhala, P., Medicherla, K.M. et al. Characterization and genome-wide sequence analysis of an ectomycorrhizal fungus Pisolithus albus, a potential source for reclamation of degraded lands. 3 Biotech 13, 58 (2023). https://doi.org/10.1007/s13205-023-03483-5
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DOI: https://doi.org/10.1007/s13205-023-03483-5