Abstract
Environmental pollution caused by polycyclic aromatic hydrocarbons (PAHs) involves a high-risk and have received considerable attention due to their carcinogenic, teratogenic, and mutagenic properties. Phenanthrene (PHE) is a low molecular weight PAH, which has three benzene rings. It is one of the most common PAH found in contaminated environments mainly due to its low volatilization ability and hydrophobic character. A PHE degrading bacterium was isolated from an industrial contaminated soil using enrichment culture techniques. Based on macroscopic, microscopic examination and phylogenetic analysis, this bacterium was classified as Stenotrophomonas indicatrix and named strain CPHE1. Several authors have reported about bacteria stains, which can degrade PHE, but this is the first time where the ability of S. indicatrix to biodegrade and mineralize PHE has been demonstrated.
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Data availability
The sequencing data generated in this study were deposited in the National Center for Biotechnology Information (NCBI), under the BioProject ID PRJNA868539. The whole genome shotgun of S. indicatrix CPHE1 has been deposited in DDBJ/ENA/GenBank under the accession number JANQDV000000000. The version described in this paper is version JANQDV010000000. The raw data were deposited in the Sequence Read Archive (SRA) under the accession number SRR21098193.
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Acknowledgements
This work was supported by the Spanish Ministry of Economy and Competitiveness under the research project CMT2017-82472-C2-1-R (AEI/FEDER, UE) and by grants from the Spanish Ministry of Education, Culture and Sports for her FPU fellowship (FPU15/03740) and University of Seville for her Margarita Salas grant funded by the European Union’s Next Generation EU.
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ALM contributed to writing—original draft preparation, conceptualization, methodology, investigation, EM contributed to resources, funding acquisition, FM contributed to resources, supervision, JLGP contributed to date curation, JV contributed to investigation, validation, supervision, writing.
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Lara-Moreno, A., Morillo, E., Merchán, F. et al. Genome sequence of Stenotrophomonas indicatrix CPHE1, a powerful phenanthrene-degrading bacterium. 3 Biotech 13, 53 (2023). https://doi.org/10.1007/s13205-023-03469-3
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DOI: https://doi.org/10.1007/s13205-023-03469-3