Abstract
Fourteen Streptomyces strains reported earlier as plant growth promoters (PGP) in chickpea were characterized for production of ammonia and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase and solubilization of silica and zinc. The results showed that nine (CAI-17, CAI-78, KAI-26, CAI-21, CAI-26, MMA-32, CAI-140, CAI-155 and KAI-180) and six (CAI-17, CAI-21, CAI-26, CAI-13, CAI-93 and KAI-180) strains were found to produce ammonia and ACC deaminase, respectively, while one (KAI-180) and eight (CAI-17, CAI-21, CAI-26, MMA-32, CAI-13, CAI-85, CAI-93 and KAI-180) strains solubilized silica and zinc, respectively. The selected 14 Streptomyces strains were categorized into three consortia groups, consortium-1 (CAI-17, CAI-68, CAI-78, KAI-26 and KAI-27), consortium-2 (CAI-21, CAI-26 and MMA-32) and consortium-3 (CAI-13, CAI-85, CAI-93, CAI-140, CAI-155 and KAI-180), based on their compatibility, and evaluated for their PGP traits in chickpea. The experiment was conducted under field conditions with two chickpea varieties over two years. The consortia-treated plots enhanced nodule number up to 23%, nodule weight up to 36%, root weight up to 27% and shoot weight up to 26% at 30 days after sowing and pod weight up to 35%, pod number up to 34% and grain yield up to 24% at harvest over the un-inoculated control plots. The harvested grains of consortia treatments were found to enhance crude protein up to 14%, crude fibre up to 17% and crude fat up to 16% over the grains from un-inoculated control. The rhizosphere soils of the consortia-treated plots enhanced total nitrogen up to 21%, organic carbon up to 8% and available phosphorous up to 16% over the un-inoculated control plots. This investigation demonstrated the potential use of the selected consortium of Streptomyces strains in the farmers’ fields to improve the chickpea yields and soil fertility.
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Abbreviations
- PGP:
-
Plant growth promotion
- PGPM:
-
Plant growth-promoting microorganisms
- ACC:
-
1-Aminocyclopropane-1-carboxylate
- SCB:
-
Starch casein broth
- DAS:
-
Days after sowing
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This work has been undertaken as part of the CGIAR Research Program on Grain Legumes and Dry Land Cereals.
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SG and SS designed, supervised and finalized the experiments; VS and UC completed the experiments; SG wrote the original draft and SP, VS, UC and SS reviewed and finalized the manuscript.
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Gopalakrishnan, S., Srinivas, V., Chand, U. et al. Streptomyces consortia-mediated plant growth-promotion and yield performance in chickpea. 3 Biotech 12, 318 (2022). https://doi.org/10.1007/s13205-022-03389-8
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DOI: https://doi.org/10.1007/s13205-022-03389-8
Keywords
- Streptomyces
- Consortia
- Plant growth promotion
- Chickpea
- Soil health