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Safety evaluation of mycotoxin citrinin production from Monascus ruber through whole-genome sequencing and analytical evaluation

Abstract

In this study, the whole genome of Monascus ruber KACC 46666 was generated using the PacBio RSII sequencer with high-quality de novo assembly to obtain trustworthy assembly and annotation using genome assemblies with long reads from PacBio single-molecule real-time sequencing. The whole genome of M. ruber has a total length of 25.9 Mb, divided in 13 contigs with 9639 genes. The functions of genes involved in secondary metabolite production were further analyzed. Gene clusters involved in the production of Monascus pigment, monacolin K, and mycotoxin citrinin were identified. Notably, most of the citrinin gene cluster was lost, as confirmed via high-performance liquid chromatography analysis. This genome-level safety evaluation of industrially important Monascus strains will provide valuable information for genome-based microbial engineering of natural food colorants and production of commercially important secondary metabolites such as monacolin K.

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Data availability

The whole-genome sequence of a wild-type M. ruber strain (KACC 46666) was deposited in the National Center for Biotechnology Information (NCBI) database under the accession number JAMZNN000000000 (BioProject accession code PRJNA749921). The raw sequencing data are available at the Sequence Read Archive database of NCBI (accessions: SRX11561645-SRX11561649).

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Acknowledgements

This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education [NRF-2021R1F1A1058927] and the Ministry of Science and ICT [NRF-2021M1A5A1075524] [KOPRI-PN22014]. The M. ruber KACC 46666 strain was kindly offered by Korean Agricultural Culture Collection (KACC).

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Authors

Contributions

Conceptualization, HRY, YK, and HJK; methodology, HRY, YK, and HJK; validation, HRY, YK, and HJK; formal analysis, HRY, YK, and HJK; data curation, HRY, YK, SH and HJK; writing—original draft preparation, HRY, YK, and HJK; writing—review and editing, SCS and HJK; visualization, HRY, YK, SH, SCS and HJK; supervision, HJK; project administration, HJK; funding acquisition, HJK.

Corresponding author

Correspondence to Hyo Jin Kim.

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Conflict of interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Yoon, H.R., Ku, D., Han, S. et al. Safety evaluation of mycotoxin citrinin production from Monascus ruber through whole-genome sequencing and analytical evaluation. 3 Biotech 12, 214 (2022). https://doi.org/10.1007/s13205-022-03287-z

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  • DOI: https://doi.org/10.1007/s13205-022-03287-z

Keywords

  • Monascus ruber
  • Whole-genome sequencing
  • Citrinin
  • Fungal metabolites