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Complete genome sequence of a multiple-stress-tolerant bacterium Halomonas piezotolerans NBT06E8T isolated from a deep-sea sediment sample of the New Britain Trench

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Abstract

Halomonas piezotolerans NBT06E8T is a Gram-stain-negative, moderately halophilic, piezotolerant, H2O2 and heavy metal-resistant bacterium, isolated from a deep-sea sediment sample collected from the New Britain Trench at depth of 8900 m. Growth of the strain was observed at 4–45 °C (optimum 30 °C), at pH 5–11 (optimum 8–9) and in 0.5–21% (w/v) NaCl (optimum 3–7%). The optimum pressure for growth was 0.1–30 MPa (megapascal) with tolerance up to 60 MPa. Under optimum growth conditions, the strain could tolerant 15 mM H2O2. Here, we report the complete genome of H. piezotolerans NBT06E8T, which consists of 3,945,801 bp (G + C content of 57.93%) with a single chromosome, 3509 protein-coding genes, 60 tRNAs and 6 rRNA operons. Genomic analysis revealed the capability of utilizing various carbon and nitrogen sources, the presence of multiple toxin–antitoxin systems and strain-specific type VI secretion system benefitting its adaptation to the oligotrophic hadal environments. Multiple respiratory chain components, especially the strain-specific anaerobic enzymes, could allow its survival in both surficial and buried sediments with variable oxygen concentrations. Gene function and metabolic pathway analysis showed that strain NBT06E8T encodes a series of genes related to high hydrostatic pressure tolerance, antioxidative stress and heavy metal resistance, which could also contribute to its deep-sea adaptation strategies. The complete genome sequence of H. piezotolerans NBT06E8T provides further insights into the stress adaptation strategies of deep-sea bacteria and potential biotechnological application of Halomonas species.

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Acknowledgements

We thank all the crew and on-board scientists of M/V Zhangjian for collecting the sediment samples in the New Britain Trench in January 2017. This study was funded by the Laboratory for Marine Biology and Biotechnology (Grant No. OF2019NO06), the Pilot National Laboratory for Marine Science and Technology (Qingdao) and the National Natural Science Foundation of China (Grant No. 91951210).

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Author notes

  1. Jiahua Wang, Zhe Xie and Ying Liu have contribute equally to this work and are the co-first authors.

Authors and Affiliations

  1. Shanghai Engineering Research Center of Hadal Science and Technology, College of Marine Sciences, Shanghai Ocean University, Shanghai, 201306, People’s Republic of China

    Jiahua Wang, Zhe Xie, Ying Liu, Fangfang Yan, Junwei Cao, Rulong Liu, Li Wang, Yuli Wei & Jiasong Fang

  2. Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, 266237, People’s Republic of China

    Zhe Xie & Jiasong Fang

  3. National Engineering Research Center for Oceanic Fisheries, Shanghai Ocean University, Shanghai, 201306, People’s Republic of China

    Junwei Cao, Rulong Liu & Li Wang

  4. Department of Natural Sciences, Hawaii Pacific University, Honolulu, HI, 96813, USA

    Jiasong Fang

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  1. Jiahua Wang
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Contributions

Conceptualization, ZX and JW; investigation, JW, ZX, YL, and FY; resources, JF; writing and original draft preparation, ZX, JW, and YL; writing, review and editing, JW, ZX, YL, JF, FY, JC, RL, and LW; project administration, JF.

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Correspondence to Zhe Xie or Jiasong Fang.

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Wang, J., Xie, Z., Liu, Y. et al. Complete genome sequence of a multiple-stress-tolerant bacterium Halomonas piezotolerans NBT06E8T isolated from a deep-sea sediment sample of the New Britain Trench. 3 Biotech 12, 236 (2022). https://doi.org/10.1007/s13205-022-03283-3

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