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Nephroprotective effect of polyphenol-rich extract of Costus spicatus in cisplatin-induced nephrotoxicity in Wistar albino rats

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Abstract

Costus spicatus (spiked spiralflag ginger) is traditionally utilised for its advantages such as antidiabetic, antihyperlipidemic, diuretic, antimicrobial, and anticancer properties. However, there is no scientific evidence on the nephroprotective potential of this plant. Thus, this study tested the nephroprotective effect of the polyphenol-rich extract of Costus spicatus leaves (PCSL) using preclinical models, including the HeK cell line and Wistar albino rats against cisplatin-induced toxicity. It also determined the polyphenolic compounds using high-performance thin-layer chromatography (HPTLC). PCSL showed significant (p < 0.05) nephroprotective potential against cisplatin-induced nephrotoxicity in HeK cells. Moreover, in vivo studies revealed significant (p < 0.05) amelioration in serum biochemical markers and antioxidant enzymes against cisplatin-induced nephrotoxicity. PCSL significantly inhibited the level of inflammatory cytokines such as TNF-α, IL-6, and IL-1β. Moreover, PCSL restored the damage of the kidney tissues and ameliorated interstitial haemorrhage, congestion in capillaries, inflammatory cell infiltration, vacuolated cytoplasm, and tubular epithelial injury with widened Bowman's space. In addition, HPTLC analysis revealed that PCSL comprised polyphenolic compounds such as caffeic acid, quercetin, and ferulic acid. In conclusion, PCSL exerted nephroprotective potential by modulating the expression of inflammation, oxidative stress, and histological architecture of kidney tissues.

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Abbreviations

PCSL:

Polyphenolic enriched extract of Costus spicatus leaves

HPTLC:

High-performance thin-layer chromatography

CKD:

Chronic kidney disease

OS:

Oxidative stress

MTT:

(3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide)

DMEM:

Dulbecco's Modified Eagle Medium

PBS:

Phosphate buffer saline

TNF-α:

Tumour necrosis factor

IL-6:

Interleukin 6

IL-1β:

Interleukin -1β

SOD:

Superoxide dismutase

CAT:

Catalase

GPx:

Glutathione peroxidase

GAE:

Gallic acid equivalent

MDA:

Malondialdehyde

NCSS:

National Centre For Cell Science

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Acknowledgements

Authors are thankful to the Taif University Researchers Supporting Project (Number TURSP-2020/124), Taif University, Taif, Saudi Arabia for supporting this work.

Funding

This research was funded by Taif University Research Supporting Project number “TURSP-2020/124”.

Author information

Authors and Affiliations

  1. Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia

    Amena Ali

  2. Department of Pharmacognosy, College of Pharmacy, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia

    Abuzer Ali

  3. Department of Pharmacy, Mohammed Al-Mana College for Medical Sciences, Dammam, 34222, Saudi Arabia

    Wasim Ahmad

  4. Department of Natural Products and Alternative Medicine, College of Clinical Pharmacy, Imam Abdul Rahman Bin Faisal University, Dammam, 31441, Saudi Arabia

    Mohd Amir

  5. Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Cawangan Selangor, Kampus Puncak Alam, 42300, Bandar Puncak Alam, Selangor Darul Ehsan, Malaysia

    Kamran Ashraf

  6. Department of Pharmacognosy, College of Pharmacy, King Khalid University, Abha, Saudi Arabia

    Shadma Wahab

  7. Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj, Saudi Arabia

    Prawez Alam

  8. Hakikullah Chaudhary College of Pharmacy, Gharighat, Gonda, 271312, U.P, India

    Abutahir

  9. Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, 110062, India

    Adil Ahamad

Authors
  1. Amena Ali
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  2. Abuzer Ali
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  3. Wasim Ahmad
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  4. Mohd Amir
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  5. Kamran Ashraf
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  6. Shadma Wahab
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  7. Prawez Alam
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  8. Abutahir
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  9. Adil Ahamad
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Contributions

The authors confirm their contribution as follows: Study conception and design: AA, ABA (Abuzer Ali), and WA; data collection and analysis: WA, MA, and KA; interpretation of results: WA, PA, A AH (Adil Ahamad) and AA; draft manuscript preparation: MA, ABA (Abuze Ali), KA, SW, AT, PA and A AH (Adil Ahamad). All authors reviewed the results and approved the final version of the manuscript.

Corresponding author

Correspondence to Amena Ali.

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Conflict of interest

The authors declare no conflict of interest.

Ethical approval

The study was approved by Ethics Committee (43–101), Taif University, Saudi Arabia.

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Ali, A., Ali, A., Ahmad, W. et al. Nephroprotective effect of polyphenol-rich extract of Costus spicatus in cisplatin-induced nephrotoxicity in Wistar albino rats. 3 Biotech 12, 189 (2022). https://doi.org/10.1007/s13205-022-03233-z

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